Heavy Metal Transporters-Associated Proteins in Solanum tuberosum: Genome-Wide Identification, Comprehensive Gene Feature, Evolution and Expression Analysis
Abstract
:1. Introduction
2. Results
2.1. Genome-Wide Identification, Chromosome Assignment, and Collinearity Analysis
2.2. Multiple Sequence Alignment, Phylogenetic Analysis, and Classification
2.3. Analysis of Conserved Motifs and Gene Structure
2.4. Expression Profiles in Various Tissues and Organs
2.5. Analysis of Abiotic Stress-Related Cis-Acting Elements and Expression Pattern of Partial StHMA Members
3. Discussion
4. Materials and Methods
4.1. Plant Growth Conditions and Treatments
4.2. RNA Extraction and Quality Control
4.3. Identification, Physicochemical Properties Analysis, Chromosome Distribution Characteristics and Collinearity Analysis of the HMA Gene Family in S. tuberosum
4.4. Multi-Sequence Alignment of HMA Gene Family and Phylogenetic Tree Construction in S. tuberosum
4.5. Genetic Structure and Motif Analysis of the HMA Gene Family in S. tuberosum
4.6. Cis-Element and Expression Analysis of StHMA Gene Family
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
ABC | ATP-binding cassette |
CAX | cation/H+ antiporters |
CET | cation-efflux transporter |
HAD | Haloacid dehalogenase |
HMA | Heavy metal ATPase |
IRT | iron transporter |
LCT | low-affinity cation transporter |
MBD | metal binding domains |
MTP | metal tolerance protein |
NRAMP | natural resistance associated macrophage protein |
ZIP | Zn-regulated transporter-like protein |
NJ | Neighbor-joining |
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He, G.; Qin, L.; Tian, W.; Meng, L.; He, T.; Zhao, D. Heavy Metal Transporters-Associated Proteins in Solanum tuberosum: Genome-Wide Identification, Comprehensive Gene Feature, Evolution and Expression Analysis. Genes 2020, 11, 1269. https://doi.org/10.3390/genes11111269
He G, Qin L, Tian W, Meng L, He T, Zhao D. Heavy Metal Transporters-Associated Proteins in Solanum tuberosum: Genome-Wide Identification, Comprehensive Gene Feature, Evolution and Expression Analysis. Genes. 2020; 11(11):1269. https://doi.org/10.3390/genes11111269
Chicago/Turabian StyleHe, Guandi, Lijun Qin, Weijun Tian, Lulu Meng, Tengbing He, and Degang Zhao. 2020. "Heavy Metal Transporters-Associated Proteins in Solanum tuberosum: Genome-Wide Identification, Comprehensive Gene Feature, Evolution and Expression Analysis" Genes 11, no. 11: 1269. https://doi.org/10.3390/genes11111269