Expression of Genes Encoding Manganese Peroxidase and Laccase of Ganoderma boninense in Response to Nitrogen Sources, Hydrogen Peroxide and Phytohormones
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of Fungal Cultures
2.2. Enzyme Assays
2.3. Inoculation of Oil Palms with G. boninense in the Presence of Different Nitrogen Sources
2.4. Primer Design from Partial Transcripts
2.5. Total RNA Extraction and RACE-PCR
2.6. Sequence Analysis of Full-Length cDNA
2.7. Quantitative Reverse Transcription-PCR (qRT-PCR)
3. Results and Discussion
3.1. Sequence Analysis of GbMnPs and GbLacs in G. boninense
3.2. Enzyme Activities of MnP and Lac in G. boninense Treated with Different Nitrogen Sources, Phytohormones and H2O2
3.3. Transcript Abundance of MnPs and Lacs in G. boninense Treated with Different Nitrogen Sources, Phytohormones and H2O2
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Ho, P.-Y.; Namasivayam, P.; Sundram, S.; Ho, C.-L. Expression of Genes Encoding Manganese Peroxidase and Laccase of Ganoderma boninense in Response to Nitrogen Sources, Hydrogen Peroxide and Phytohormones. Genes 2020, 11, 1263. https://doi.org/10.3390/genes11111263
Ho P-Y, Namasivayam P, Sundram S, Ho C-L. Expression of Genes Encoding Manganese Peroxidase and Laccase of Ganoderma boninense in Response to Nitrogen Sources, Hydrogen Peroxide and Phytohormones. Genes. 2020; 11(11):1263. https://doi.org/10.3390/genes11111263
Chicago/Turabian StyleHo, Pei-Yin, Parameswari Namasivayam, Shamala Sundram, and Chai-Ling Ho. 2020. "Expression of Genes Encoding Manganese Peroxidase and Laccase of Ganoderma boninense in Response to Nitrogen Sources, Hydrogen Peroxide and Phytohormones" Genes 11, no. 11: 1263. https://doi.org/10.3390/genes11111263