Novel Regulators of Sugar-Mediated Lateral Root Development in Arabidopsis thaliana
1
State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Xianyang 712100, Shaanxi, China
2
Department of Biological Science, Florida State University, Tallahassee, FL 32306, USA
*
Author to whom correspondence should be addressed.
Genes 2020, 11(11), 1257; https://doi.org/10.3390/genes11111257
Received: 15 September 2020
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Revised: 5 October 2020
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Accepted: 23 October 2020
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Published: 25 October 2020
(This article belongs to the Special Issue Genes at Ten)
Lateral root development is a complex process regulated by numerous factors. An important role for sugar in lateral root development has been known for a while, but the underlying molecular basis still remains unclear. In this study, we first showed that WOX7, a sugar-inducible negative regulator of lateral root development, acts downstream of the glucose sensor HXK1. Using a transgenic line homozygous for a transgene expressing GFP under the control of the WOX7 promoter, we next performed a genetic screen to identify additional genes in this development pathway. A number of mutants with altered level of WOX7 expression were recovered, and two with increased WOX7 expression, named ewe-1 and ewe-2 (for Enhanced WOX7 Expression), were further characterized. Both mutants manifest delayed lateral root development, and genetic analysis indicates that single recessive mutations are responsible for the observed phenotypes. The mutations were then located to similar regions on chromosome 2 by marker-assisted analyses, and candidate genes were identified through whole genome sequencing. The significance and limitations of this work are discussed.
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Keywords:
lateral root; sugar; EMS mutagenesis; mapping; WOX7; HXK1
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MDPI and ACS Style
Li, J.; Wang, B.; Zhu, X.; Li, R.; Fu, J.; Cui, H. Novel Regulators of Sugar-Mediated Lateral Root Development in Arabidopsis thaliana. Genes 2020, 11, 1257. https://doi.org/10.3390/genes11111257
AMA Style
Li J, Wang B, Zhu X, Li R, Fu J, Cui H. Novel Regulators of Sugar-Mediated Lateral Root Development in Arabidopsis thaliana. Genes. 2020; 11(11):1257. https://doi.org/10.3390/genes11111257
Chicago/Turabian StyleLi, Jinzhu, Bingxin Wang, Xinxing Zhu, Rong Li, Jing Fu, and Hongchang Cui. 2020. "Novel Regulators of Sugar-Mediated Lateral Root Development in Arabidopsis thaliana" Genes 11, no. 11: 1257. https://doi.org/10.3390/genes11111257
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