Next Article in Journal
Variable Number Tandem Repeats in the Mitochondrial DNA of Lentinula edodes
Previous Article in Journal
The Caribou (Rangifer tarandus) Genome
Previous Article in Special Issue
DNA Methylation Analysis of the Citrullus lanatus Response to Cucumber Green Mottle Mosaic Virus Infection by Whole-Genome Bisulfite Sequencing
Open AccessArticle

The CaAP2/ERF064 Regulates Dual Functions in Pepper: Plant Cell Death and Resistance to Phytophthora capsici

1
College of Horticulture, Northwest A&F University, Yangling 712100, China
2
Tianjin Vegetable Research Center, Tianjin 300192, China
3
Xuhuai Region Huaiyin Institute of Agricultural Sciences, Huai’an 223001, China
*
Author to whom correspondence should be addressed.
Genes 2019, 10(7), 541; https://doi.org/10.3390/genes10070541
Received: 9 May 2019 / Revised: 15 July 2019 / Accepted: 15 July 2019 / Published: 17 July 2019
(This article belongs to the Special Issue Genetics and Epigenetics of Biotic Stress Response in Plants)
  |  
PDF [8692 KB, uploaded 17 July 2019]
  |  

Abstract

Phytophthora blight is one of the most destructive diseases of pepper (Capsicum annuum L.) globally. The APETALA2/Ethylene Responsive Factors (AP2/ERF) genes play a crucial role in plant response to biotic stresses but, to date, have not been studied in the context of Phytophthora resistance in pepper. Here, we documented potential roles for the pepper CaAP2/ERF064 gene in inducing cell death and conferring resistance to Phytophthora capsici (P. capsici) infection. Results revealed that the N-terminal, AP2 domain, and C-terminal of CaAP2/ERF064 protein is responsible for triggering cell death in Nicotiana benthamiana (N. benthamiana). Moreover, the transcription of CaAP2/ERF064 in plant is synergistically regulated by the Methyl-Jasmonate (MeJA) and ethephon (ET) signaling pathway. CaAP2/ERF064 was found to regulate the expression of CaBPR1, which is a pathogenesis-related (PR) gene of pepper. Furthermore, the silencing of CaAP2/ERF064 compromised the pepper plant resistance to P. capsici by reducing the transcript level of defense-related genes CaBPR1, CaPO2, and CaSAR82, while the ectopic expression of CaAP2/ERF064 in N. benthamiana plant elevated the expression level of NbPR1b and enhanced resistance to P. capsici. These results suggest that CaAP2/ERF064 could positively regulate the defense response against P. capsici by modulating the transcription of PR genes in the plant. View Full-Text
Keywords: pepper; AP2/ERF; P. capsici; cell death; PR gene pepper; AP2/ERF; P. capsici; cell death; PR gene
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Jin, J.-H.; Zhang, H.-X.; Ali, M.; Wei, A.-M.; Luo, D.-X.; Gong, Z.-H. The CaAP2/ERF064 Regulates Dual Functions in Pepper: Plant Cell Death and Resistance to Phytophthora capsici. Genes 2019, 10, 541.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Genes EISSN 2073-4425 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top