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Variable Number Tandem Repeats in the Mitochondrial DNA of Lentinula edodes

1
Division of Applied Life Science and Research Institute of Life Sciences, Gyeongsang National University, Jinju 52828, Korea
2
Department of Biology, Chungbuk National University, Cheongju 28644, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Genes 2019, 10(7), 542; https://doi.org/10.3390/genes10070542
Received: 26 June 2019 / Revised: 13 July 2019 / Accepted: 15 July 2019 / Published: 17 July 2019
(This article belongs to the Section Microbial Genetics and Genomics)
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Abstract

Variable number tandem repeats (VNTRs) in mitochondrial DNA (mtDNA) of Lentinula edodes are of interest for their role in mtDNA variation and their application as genetic marker. Sequence analysis of three L. edodes mtDNAs revealed the presence of VNTRs of two categories. Type I VNTRs consist of two types of repeat units in a symmetric distribution, whereas Type II VNTRs contain tandemly arrayed repeats of 7- or 17-bp DNA sequences. The number of repeat units was variable depending on the mtDNA of different strains. Using the variations in VNTRs as a mitochondrial marker and the A mating type as a nuclear type marker, we demonstrated that one of the two nuclei in the donor dikaryon preferentially enters into the monokaryotic cytoplasm to establish a new dikaryon which still retains the mitochondria of the monokaryon in the individual mating. Interestingly, we found 6 VNTRs with newly added repeat units from the 22 mates, indicating that elongation of VNTRs occurs during replication of mtDNA. This, together with comparative analysis of the repeating pattern, enables us to propose a mechanistic model that explains the elongation of Type I VNTRs through reciprocal incorporation of basic repeat units, 5’-TCCCTTTAGGG-3’ and its complementary sequence (5’-CCCTAAAGGGA-3’). View Full-Text
Keywords: Lentinula edodes; mating; mitochondrial DNA; tandem repeats; VNTR Lentinula edodes; mating; mitochondrial DNA; tandem repeats; VNTR
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Kim, S.; Song, Y.; Ha, B.; Moon, Y.J.; Kim, M.; Ryu, H.; Ro, H.-S. Variable Number Tandem Repeats in the Mitochondrial DNA of Lentinula edodes. Genes 2019, 10, 542.

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