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Article

Diversity of “Ca. Micrarchaeota” in Two Distinct Types of Acidic Environments and Their Associations with Thermoplasmatales

1
School of Natural Sciences, Bangor University, Deiniol Rd, Bangor LL57 2UW, UK
2
Centre for Environmental Biotechnology, Bangor University, Deiniol Rd, Bangor LL57 2UW, UK
3
Winogradsky Institute of Microbiology, Research Center for Biotechnology, Russian Academy of Sciences, Prospect 60-Letiya Oktyabrya 7/2, Moscow 117312, Russia
4
National Research Center “Kurchatov Institute”, Akademika Kurchatova sq., 1, Moscow 123182, Russia
*
Author to whom correspondence should be addressed.
Genes 2019, 10(6), 461; https://doi.org/10.3390/genes10060461
Received: 16 April 2019 / Revised: 30 May 2019 / Accepted: 11 June 2019 / Published: 15 June 2019
(This article belongs to the Special Issue Genetics and Genomics of Acidophiles)
Candidatus Micrarchaeota” are widely distributed in acidic environments; however, their cultivability and our understanding of their interactions with potential hosts are very limited. Their habitats were so far attributed with acidic sites, soils, peats, freshwater systems, and hypersaline mats. Using cultivation and culture-independent approaches (16S rRNA gene clonal libraries, high-throughput amplicon sequencing of V3-V4 region of 16S rRNA genes), we surveyed the occurrence of these archaea in geothermal areas on Kamchatka Peninsula and Kunashir Island and assessed their taxonomic diversity in relation with another type of low-pH environment, acid mine drainage stream (Wales, UK). We detected “Ca. Micrarchaeota” in thermophilic heterotrophic enrichment cultures of Kunashir and Kamchatka that appeared as two different phylotypes, namely “Ca. Mancarchaeum acidiphilum”-, and ARMAN-2-related, alongside their potential hosts, Cuniculiplasma spp. and other Thermoplasmatales archaea without defined taxonomic position. These clusters of “Ca. Micrarchaeota” together with three other groups were also present in mesophilic acid mine drainage community. Present work expands our knowledge on the diversity of “Ca. Micrarchaeota” in thermophilic and mesophilic acidic environments, suggests cultivability patterns of acidophilic archaea and establishes potential links between low-abundance species of thermophilic “Ca. Micrarchaeota” and certain Thermoplasmatales, such as Cuniculiplasma spp. in situ. View Full-Text
Keywords: Ca. Mancarchaeum acidiphilum”; ARMAN-2; “Ca. Micrarchaeota”; DPANN superphylum; Thermoplasmatales; Cuniculiplasma; acidic environments; acid mine drainage sites; terrestrial hot springs Ca. Mancarchaeum acidiphilum”; ARMAN-2; Ca. Micrarchaeota”; DPANN superphylum; Thermoplasmatales; Cuniculiplasma; acidic environments; acid mine drainage sites; terrestrial hot springs
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MDPI and ACS Style

Golyshina, O.V.; Bargiela, R.; Toshchakov, S.V.; Chernyh, N.A.; Ramayah, S.; Korzhenkov, A.A.; Kublanov, I.V.; Golyshin, P.N. Diversity of “Ca. Micrarchaeota” in Two Distinct Types of Acidic Environments and Their Associations with Thermoplasmatales. Genes 2019, 10, 461. https://doi.org/10.3390/genes10060461

AMA Style

Golyshina OV, Bargiela R, Toshchakov SV, Chernyh NA, Ramayah S, Korzhenkov AA, Kublanov IV, Golyshin PN. Diversity of “Ca. Micrarchaeota” in Two Distinct Types of Acidic Environments and Their Associations with Thermoplasmatales. Genes. 2019; 10(6):461. https://doi.org/10.3390/genes10060461

Chicago/Turabian Style

Golyshina, Olga V., Rafael Bargiela, Stepan V. Toshchakov, Nikolay A. Chernyh, Soshila Ramayah, Aleksei A. Korzhenkov, Ilya V. Kublanov, and Peter N. Golyshin 2019. "Diversity of “Ca. Micrarchaeota” in Two Distinct Types of Acidic Environments and Their Associations with Thermoplasmatales" Genes 10, no. 6: 461. https://doi.org/10.3390/genes10060461

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