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Open AccessArticle

RNA Sequencing Analysis of Chicken Cecum Tissues Following Eimeria tenella Infection in Vivo

1
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China
2
Animal Husbandry and Veterinary Station of Kunshan City, Kunshan 215300, Jiangsu, China
3
Jiangsu Jinghai Poultry Group Co., Ltd., Haimen 226100, Jiangsu, China
*
Author to whom correspondence should be addressed.
Genes 2019, 10(6), 420; https://doi.org/10.3390/genes10060420
Received: 29 April 2019 / Revised: 28 May 2019 / Accepted: 29 May 2019 / Published: 31 May 2019
(This article belongs to the Section Animal Genetics and Genomics)
Eimeria tenella (E. tenella) is one of the most frequent and pathogenic species of protozoan parasites of the genus Eimeria that exclusively occupies the cecum, exerting a high economic impact on the poultry industry. To investigate differentially expressed genes (DEGs) in the cecal tissue of Jinghai yellow chickens infected with E. tenella, the molecular response process, and the immune response mechanism during coccidial infection, RNA-seq was used to analyze the cecal tissues of an E. tenella infection group (JS) and an uninfected group (JC) on the seventh day post-infection. The DEGs were screened by functional and pathway enrichment analyses. The results indicated that there were 5477 DEGs (p-value < 0.05) between the JS and the JC groups, of which 2942 were upregulated, and 2535 were downregulated. GO analysis indicated that the top 30 significantly enriched GO terms mainly involved signal transduction, angiogenesis, inflammatory response, and blood vessel development. KEGG analysis revealed that the top significantly enriched signaling pathways included focal adhesion, extracellular matrix–receptor interaction, and peroxisome proliferator-activated receptor. The key DEGs in these pathways included ANGPTL4, ACSL5, VEGFC, MAPK10, and CD44. These genes play an important role in the infection of E. tenella. This study further enhances our understanding of the molecular mechanism of E. tenella infection in chickens. View Full-Text
Keywords: RNA-seq; E. tenella; chicken; cecum; differentially expressed genes RNA-seq; E. tenella; chicken; cecum; differentially expressed genes
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Wang, X.; Zou, W.; Yu, H.; Lin, Y.; Dai, G.; Zhang, T.; Zhang, G.; Xie, K.; Wang, J.; Shi, H. RNA Sequencing Analysis of Chicken Cecum Tissues Following Eimeria tenella Infection in Vivo. Genes 2019, 10, 420.

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