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Volume 10
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10.3390/genes10030212
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Article

RNA Sequencing Reveals Specific Transcriptomic Signatures Distinguishing Effects of the [SWI+] Prion and SWI1 Deletion in Yeast Saccharomyces cerevisiae

by
Yury V. Malovichko
1,2,
Kirill S. Antonets
1,2,
Anna R. Maslova
2,
Elena A. Andreeva
2,3,
Sergey G. Inge-Vechtomov
2,3 and
Anton A. Nizhnikov
1,2,*
1
Laboratory for Proteomics of Supra-Organismal Systems, All-Russia Research Institute for Agricultural Microbiology (ARRIAM), Podbelskogo sh., 3, Pushkin, 196608 St. Petersburg, Russia
2
Department of Genetics and Biotechnology, St. Petersburg State University, Universitetskaya nab., 7/9, 199034 St. Petersburg, Russia
3
Vavilov Institute of General Genetics, St. Petersburg Branch, Universitetskaya nab., 7/9, 199034 St. Petersburg, Russia
*
Author to whom correspondence should be addressed.
Genes 2019, 10(3), 212; https://doi.org/10.3390/genes10030212
Received: 10 February 2019 / Revised: 1 March 2019 / Accepted: 8 March 2019 / Published: 12 March 2019
(This article belongs to the Section Microbial Genetics and Genomics)
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Abstract

Prions are infectious, self-perpetuating protein conformers. In mammals, pathological aggregation of the prion protein causes incurable neurodegenerative disorders, while in yeast Saccharomyces cerevisiae, prion formation may be neutral or even beneficial. According to the prevailing contemporary point of view, prion formation is considered to be a functional inactivation of the corresponding protein whose conformational state shifts from the functional monomeric one to the infectious aggregated one. The Swi1 protein forms the [SWI+] prion and belongs to the nucleosome remodeler complex SWI/SNF controlling the expression of a significant part of the yeast genome. In this work, we performed RNA sequencing of isogenic S. cerevisiae strains grown on the media containing galactose as the sole carbon source. These strains bore the [SWI+] prion or had its structural gene SWI1 deleted. The comparative analysis showed that [SWI+] affects genome expression significantly weaker as compared to the SWI1 deletion. Moreover, in contrast to [SWI+], the SWI1 deletion causes the general inhibition of translation-related genes expression and chromosome I disomy. At the same time, the [SWI+] prion exhibits a specific pattern of modulation of the metabolic pathways and some biological processes and functions, as well as the expression of several genes. Thus, the [SWI+] prion only partially corresponds to the loss-of-function of SWI1 and demonstrates several gain-of-function traits. View Full-Text
Keywords: prion; amyloid; yeast; S. cerevisiae; transcriptome; RNA-Seq; Swi1; [SWI+]; SWI/SNF prion; amyloid; yeast; S. cerevisiae; transcriptome; RNA-Seq; Swi1; [SWI+]; SWI/SNF
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MDPI and ACS Style

Malovichko, Y.V.; Antonets, K.S.; Maslova, A.R.; Andreeva, E.A.; Inge-Vechtomov, S.G.; Nizhnikov, A.A. RNA Sequencing Reveals Specific Transcriptomic Signatures Distinguishing Effects of the [SWI+] Prion and SWI1 Deletion in Yeast Saccharomyces cerevisiae. Genes 2019, 10, 212. https://doi.org/10.3390/genes10030212

AMA Style

Malovichko YV, Antonets KS, Maslova AR, Andreeva EA, Inge-Vechtomov SG, Nizhnikov AA. RNA Sequencing Reveals Specific Transcriptomic Signatures Distinguishing Effects of the [SWI+] Prion and SWI1 Deletion in Yeast Saccharomyces cerevisiae. Genes. 2019; 10(3):212. https://doi.org/10.3390/genes10030212

Chicago/Turabian Style

Malovichko, Yury V., Kirill S. Antonets, Anna R. Maslova, Elena A. Andreeva, Sergey G. Inge-Vechtomov, and Anton A. Nizhnikov. 2019. "RNA Sequencing Reveals Specific Transcriptomic Signatures Distinguishing Effects of the [SWI+] Prion and SWI1 Deletion in Yeast Saccharomyces cerevisiae" Genes 10, no. 3: 212. https://doi.org/10.3390/genes10030212

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