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10.3390/genes10020129
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Communication

Transcriptome Analysis of Male and Female Mature Gonads of Silver Sillago (Sillago sihama)

by
Changxu Tian
,
Zhiyuan Li
,
Zhongdian Dong
,
Yang Huang
,
Tao Du
,
Huapu Chen
,
Dongneng Jiang
,
Siping Deng
,
Yulei Zhang
,
Saetan Wanida
,
Hongjuan Shi
,
Tianli Wu
,
Chunhua Zhu
and
Guangli Li
*
Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Key Laboratory of Marine Ecology and Aquaculture Environment of Zhanjiang, Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China
*
Author to whom correspondence should be addressed.
Genes 2019, 10(2), 129; https://doi.org/10.3390/genes10020129
Submission received: 14 December 2018 / Revised: 1 February 2019 / Accepted: 7 February 2019 / Published: 11 February 2019
(This article belongs to the Section Animal Genetics and Genomics)
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Abstract

Silver sillago (Sillago sihama) is an emerging commercial marine aquaculture species in China. To date, fundamental information on S. sihama, such as genomic information, is lacking, and no data are available on the gonad transcriptome of S. sihama. Here, the first gonadal transcriptomes of S. sihama have been constructed and genes potentially involved in gonadal development and reproduction identified. Illumina sequencing generated 60.18 million clean reads for the testis and 59.10 million for the ovary. All reads were assembled into 74,038 unigenes with a mean length of 1,004 bp and N50 value of 2,190 bp. Among all the predictable unigenes, a total of 34,104 unigenes (46%) were searched against multiple databases, including 33,244 unigenes annotated in the RefSeq Non- Redundant database at NCBI, and 28,924 in Swiss-Prot. By comparing the ovary and testis, 35,367 unigenes were identified as being differentially expressed between males and females, of which 29,127 were upregulated in the testis and 6,240 were upregulated in the ovary. Numerous differentially expressed genes (DEGs) known to be involved in gonadal development and gametogenesis were identified, including amh, dmrt1, gsdf, cyp19a1a, gnrhr, and zps. Using gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses, the top 20 KEGG pathways with highest number of DEGs were found to be involved in regulating gonadal development and gametogenesis in S. sihama. Moreover, 22,666 simple sequence repeats (SSRs) were identified in 14,577 SSR-containing sequences. The findings provide a valuable dataset for future functional analyses of sex-associated genes and molecular marker assisted selection in S. sihama.
Keywords: Sillago sihama; transcriptome; gonadal development and gametogenesis; differentially expressed genes Sillago sihama; transcriptome; gonadal development and gametogenesis; differentially expressed genes

Share and Cite

MDPI and ACS Style

Tian, C.; Li, Z.; Dong, Z.; Huang, Y.; Du, T.; Chen, H.; Jiang, D.; Deng, S.; Zhang, Y.; Wanida, S.; et al. Transcriptome Analysis of Male and Female Mature Gonads of Silver Sillago (Sillago sihama). Genes 2019, 10, 129. https://doi.org/10.3390/genes10020129

AMA Style

Tian C, Li Z, Dong Z, Huang Y, Du T, Chen H, Jiang D, Deng S, Zhang Y, Wanida S, et al. Transcriptome Analysis of Male and Female Mature Gonads of Silver Sillago (Sillago sihama). Genes. 2019; 10(2):129. https://doi.org/10.3390/genes10020129

Chicago/Turabian Style

Tian, Changxu, Zhiyuan Li, Zhongdian Dong, Yang Huang, Tao Du, Huapu Chen, Dongneng Jiang, Siping Deng, Yulei Zhang, Saetan Wanida, and et al. 2019. "Transcriptome Analysis of Male and Female Mature Gonads of Silver Sillago (Sillago sihama)" Genes 10, no. 2: 129. https://doi.org/10.3390/genes10020129

APA Style

Tian, C., Li, Z., Dong, Z., Huang, Y., Du, T., Chen, H., Jiang, D., Deng, S., Zhang, Y., Wanida, S., Shi, H., Wu, T., Zhu, C., & Li, G. (2019). Transcriptome Analysis of Male and Female Mature Gonads of Silver Sillago (Sillago sihama). Genes, 10(2), 129. https://doi.org/10.3390/genes10020129

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