Complete Mitogenome of a Leaf-Mining Buprestid Beetle, Trachys auricollis, and Its Phylogenetic Implications
by
Lifang Xiao 1,2,†, Shengdi Zhang 1,†, Chengpeng Long 1, Qingyun Guo 1, Jiasheng Xu 1, Xiaohua Dai 1,2,* and Jianguo Wang 3
Lifang Xiao 1,2,†, Shengdi Zhang 1,†, Chengpeng Long 1, Qingyun Guo 1, Jiasheng Xu 1, Xiaohua Dai 1,2,* and Jianguo Wang 3
1
Leafminer Group, School of Life Sciences, Gannan Normal University, Ganzhou 341000, China
2
National Navel-Orange Engineering Research Center, Ganzhou 341000, China
3
College of Agriculture, Jiangxi Agricultural University, Nanchang 330045, China
*
Author to whom correspondence should be addressed.
†
Both authors contributed equally to this work.
Genes 2019, 10(12), 992; https://doi.org/10.3390/genes10120992
Received: 23 October 2019 / Revised: 26 November 2019 / Accepted: 28 November 2019 / Published: 1 December 2019
(This article belongs to the Special Issue Arthropod Genetics and Genomics)
A complete mitogenome of Trachys auricollis is reported, and a mitogenome-based phylogenetic tree of Elateriformia with all protein-coding genes (PCGs), rRNAs, and tRNAs is presented for the first time. The complete mitochondrial genome of T. auricollis is 16,429 bp in size and contains 13 PCGs, two rRNA genes, 22 tRNA genes, and an A + T-rich region. The A + T content of the entire genome is approximately 71.1%, and the AT skew and GC skew are 0.10 and −0.20, respectively. According to the the nonsynonymous substitution rate to synonymous substitution rates (Ka/Ks) of all PCGs, the highest and lowest evolutionary rates were observed for atp8 and cox1, respectively, which is a common finding among animals. The start codons of all PCGs are the typical ATN. Ten PCGs have complete stop codons, but three have incomplete stop codons with T or TA. As calculated based on the relative synonymous codon usage (RSCU) values, UUA(L) is the codon with the highest frequency. Except for trnS1, all 22 tRNA genes exhibit typical cloverleaf structures. The A + T-rich region of T. auricollis is located between rrnS and the trnI-trnG-trnM gene cluster, with six 72-bp tandem repeats. Both maximum likelihood (ML) and Bayesian (BI) trees suggest that Buprestoidea is close to Byrrhoidea and that Buprestoidea and Byrrhoidea are sister groups of Elateroidea, but the position of Psephenidae is undetermined. The inclusion of tRNAs might help to resolve the phylogeny of Coleoptera.
Keywords:
Buprestoidea; Byrrhoidea; Elateroidea; Scirtoidea; Elateriformia; mitochondrial genome; phylogeny
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
MDPI and ACS Style
Xiao, L.; Zhang, S.; Long, C.; Guo, Q.; Xu, J.; Dai, X.; Wang, J. Complete Mitogenome of a Leaf-Mining Buprestid Beetle, Trachys auricollis, and Its Phylogenetic Implications. Genes 2019, 10, 992.
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