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Transcriptome Analysis of the Cecal Tonsil of Jingxing Yellow Chickens Revealed the Mechanism of Differential Resistance to Salmonella

by Fei Wang 1,†, Jin Zhang 1,†, Bo Zhu 1, Jie Wang 1, Qiao Wang 1, Maiqing Zheng 1, Jie Wen 1,2, Qinghe Li 1 and Guiping Zhao 1,3,*
1
Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
2
State Key Laboratory of Animal Nutrition, Beijing 100193, China
3
School of Life Science and Engineering, Foshan University, Foshan 528000, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Genes 2019, 10(12), 979; https://doi.org/10.3390/genes10120979
Received: 9 October 2019 / Revised: 21 November 2019 / Accepted: 26 November 2019 / Published: 28 November 2019
(This article belongs to the Special Issue Domestication Genetics)
Salmonella is one of the most common food-borne pathogens. It can be transmitted between chickens, as well as to people by contaminated poultry products. In our study, we distinguished chickens with different resistances mainly based on bacterial loads. We compared the cecal tonsil transcriptomes between the susceptible and resistant chickens after Salmonella infection, aiming to identify the crucial genes participating in the antibacterial activity in the cecal tonsil. A total of 3214 differentially expressed genes (DEGs), including 2092 upregulated and 1122 downregulated genes, were identified between the two groups (fold change ≥ 2.0, padj < 0.05). Many DEGs were mainly involved in the regulation of two biological processes: crosstalk between the cecal tonsil epithelium and pathogenic bacteria, such as focal adhesion, extracellular-matrix–receptor interaction, and regulation of the actin cytoskeleton and host immune response including the cytokine–receptor interaction. In particular, the challenged resistant birds exhibited strong activation of the intestinal immune network for IgA production, which perhaps contributed to the resistance to Salmonella infection. These findings give insight into the mRNA profile of the cecal tonsil between the two groups after initial Salmonella stimulation, which may extend the known complexity of molecular mechanisms in chicken immune response to Salmonella. View Full-Text
Keywords: cecal tonsil; transcriptome; Salmonella; chicken; susceptibility cecal tonsil; transcriptome; Salmonella; chicken; susceptibility
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Wang, F.; Zhang, J.; Zhu, B.; Wang, J.; Wang, Q.; Zheng, M.; Wen, J.; Li, Q.; Zhao, G. Transcriptome Analysis of the Cecal Tonsil of Jingxing Yellow Chickens Revealed the Mechanism of Differential Resistance to Salmonella. Genes 2019, 10, 979.

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