Next Article in Journal
High Prevalence of Staphylococcus aureus Enterotoxin Gene Cluster Superantigens in Cystic Fibrosis Clinical Isolates
Next Article in Special Issue
Developmental Transcriptomic Analysis of the Cave-Dwelling Crustacean, Asellus aquaticus
Previous Article in Journal
Identification of Shoot Differentiation-Related Genes in Populus euphratica Oliv.
Previous Article in Special Issue
Identification and Description of the Key Molecular Components of the Egg Strings of the Salmon Louse (Lepeophtheirus salmonis)
Open AccessArticle

Comparative Transcriptome Analysis of Gonads for the Identification of Sex-Related Genes in Giant Freshwater Prawns (Macrobrachium Rosenbergii) Using RNA Sequencing

College of Animal Science and Technology, Guangxi University, Nanning 530001, China
Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China
Guangxi Academy of Fishery Sciences, Nanning 530021, China
College of Animal Science, Southwest University, Chongqing 402460, China
Animal Husbandry Research Institute of Guangxi Zhuang Autonomous Region, Nanning 530001, China
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Genes 2019, 10(12), 1035;
Received: 12 November 2019 / Revised: 26 November 2019 / Accepted: 2 December 2019 / Published: 11 December 2019
(This article belongs to the Special Issue Arthropod Genetics and Genomics)
The giant freshwater prawn (Macrobrachium rosenbergii) exhibits sex dimorphism between the male and female individuals. To date, the molecular mechanism governing gonadal development was unclear, and limited data were available on the gonad transcriptome of M. rosenbergii. Here, we conducted comprehensive gonadal transcriptomic analysis of female (ZW), super female (WW), and male (ZZ) M. rosenbergii for gene discovery. A total of 70.33 gigabases (Gb) of sequences were generated. There were 115,338 unigenes assembled with a mean size of 1196 base pair (bp) and N50 of 2195 bp. Alignment against the National Center for Biotechnology Information (NCBI) non-redundant nucleotide/protein sequence database (NR and NT), the Kyoto Encyclopedia of Genes and Genomes (KEGG) database, SwissProt database, Protein family (Pfam), Gene ontology (GO), and the eukaryotic orthologous group (KOG) database, 36,282 unigenes were annotated at least in one database. Comparative transcriptome analysis observed that 10,641, 16,903, and 3393 genes were significantly differentially expressed in ZW vs. ZZ, WW vs. ZZ, and WW vs. ZW samples, respectively. Enrichment analysis of differentially expressed genes (DEGs) resulted in 268, 153, and 42 significantly enriched GO terms, respectively, and a total of 56 significantly enriched KEGG pathways. Additionally, 23 putative sex-related genes, including Gtsf1, IR, HSP21, MRPINK, Mrr, and other potentially promising candidate genes were identified. Moreover, 56,241 simple sequence repeats (SSRs) were identified. Our findings provide a valuable archive for further functional analyses of sex-related genes and future discoveries of underlying molecular mechanisms of gonadal development and sex determination. View Full-Text
Keywords: Macrobrachium rosenbergii; RNA-seq; gonad; sex-related candidate gene; SSR Macrobrachium rosenbergii; RNA-seq; gonad; sex-related candidate gene; SSR
Show Figures

Figure 1

MDPI and ACS Style

Jiang, J.; Yuan, X.; Qiu, Q.; Huang, G.; Jiang, Q.; Fu, P.; Zhang, Y.; Jia, Y.; Yang, X.; Jiang, H. Comparative Transcriptome Analysis of Gonads for the Identification of Sex-Related Genes in Giant Freshwater Prawns (Macrobrachium Rosenbergii) Using RNA Sequencing. Genes 2019, 10, 1035.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop