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Volume 10
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10.3390/genes10121035
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Article

Comparative Transcriptome Analysis of Gonads for the Identification of Sex-Related Genes in Giant Freshwater Prawns (Macrobrachium Rosenbergii) Using RNA Sequencing

by
Jianping Jiang
1,2,†,
Xiang Yuan
1,†,
Qingqing Qiu
1,
Guanghua Huang
3,
Qinyang Jiang
1,
Penghui Fu
1,4,
Yu Zhang
1,
Yinhai Jia
5,
Xiurong Yang
1,* and
Hesheng Jiang
1,*
1
College of Animal Science and Technology, Guangxi University, Nanning 530001, China
2
Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China
3
Guangxi Academy of Fishery Sciences, Nanning 530021, China
4
College of Animal Science, Southwest University, Chongqing 402460, China
5
Animal Husbandry Research Institute of Guangxi Zhuang Autonomous Region, Nanning 530001, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Genes 2019, 10(12), 1035; https://doi.org/10.3390/genes10121035
Received: 12 November 2019 / Revised: 26 November 2019 / Accepted: 2 December 2019 / Published: 11 December 2019
(This article belongs to the Special Issue Arthropod Genetics and Genomics)
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Abstract

The giant freshwater prawn (Macrobrachium rosenbergii) exhibits sex dimorphism between the male and female individuals. To date, the molecular mechanism governing gonadal development was unclear, and limited data were available on the gonad transcriptome of M. rosenbergii. Here, we conducted comprehensive gonadal transcriptomic analysis of female (ZW), super female (WW), and male (ZZ) M. rosenbergii for gene discovery. A total of 70.33 gigabases (Gb) of sequences were generated. There were 115,338 unigenes assembled with a mean size of 1196 base pair (bp) and N50 of 2195 bp. Alignment against the National Center for Biotechnology Information (NCBI) non-redundant nucleotide/protein sequence database (NR and NT), the Kyoto Encyclopedia of Genes and Genomes (KEGG) database, SwissProt database, Protein family (Pfam), Gene ontology (GO), and the eukaryotic orthologous group (KOG) database, 36,282 unigenes were annotated at least in one database. Comparative transcriptome analysis observed that 10,641, 16,903, and 3393 genes were significantly differentially expressed in ZW vs. ZZ, WW vs. ZZ, and WW vs. ZW samples, respectively. Enrichment analysis of differentially expressed genes (DEGs) resulted in 268, 153, and 42 significantly enriched GO terms, respectively, and a total of 56 significantly enriched KEGG pathways. Additionally, 23 putative sex-related genes, including Gtsf1, IR, HSP21, MRPINK, Mrr, and other potentially promising candidate genes were identified. Moreover, 56,241 simple sequence repeats (SSRs) were identified. Our findings provide a valuable archive for further functional analyses of sex-related genes and future discoveries of underlying molecular mechanisms of gonadal development and sex determination. View Full-Text
Keywords: Macrobrachium rosenbergii; RNA-seq; gonad; sex-related candidate gene; SSR Macrobrachium rosenbergii; RNA-seq; gonad; sex-related candidate gene; SSR
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MDPI and ACS Style

Jiang, J.; Yuan, X.; Qiu, Q.; Huang, G.; Jiang, Q.; Fu, P.; Zhang, Y.; Jia, Y.; Yang, X.; Jiang, H. Comparative Transcriptome Analysis of Gonads for the Identification of Sex-Related Genes in Giant Freshwater Prawns (Macrobrachium Rosenbergii) Using RNA Sequencing. Genes 2019, 10, 1035. https://doi.org/10.3390/genes10121035

AMA Style

Jiang J, Yuan X, Qiu Q, Huang G, Jiang Q, Fu P, Zhang Y, Jia Y, Yang X, Jiang H. Comparative Transcriptome Analysis of Gonads for the Identification of Sex-Related Genes in Giant Freshwater Prawns (Macrobrachium Rosenbergii) Using RNA Sequencing. Genes. 2019; 10(12):1035. https://doi.org/10.3390/genes10121035

Chicago/Turabian Style

Jiang, Jianping, Xiang Yuan, Qingqing Qiu, Guanghua Huang, Qinyang Jiang, Penghui Fu, Yu Zhang, Yinhai Jia, Xiurong Yang, and Hesheng Jiang. 2019. "Comparative Transcriptome Analysis of Gonads for the Identification of Sex-Related Genes in Giant Freshwater Prawns (Macrobrachium Rosenbergii) Using RNA Sequencing" Genes 10, no. 12: 1035. https://doi.org/10.3390/genes10121035

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