Differential Expression of Genes Related to Sexual Determination Can Modify the Reproductive Cycle of Astyanax scabripinnis (Characiformes: Characidae) in B Chromosome Carrier Individuals
Abstract
1. Introduction
2. Materials and Methods
2.1. Characterization of the Study Object
2.2. Cytogenetics
2.3. Histological Analysis
2.4. RNA Extraction and cDNA Synthesis
2.5. Quantitative PCR in Real-Time (qRT-PCR)
2.6. Illumina Sequencing and Coverage Analysis of Sex-Associated Genes
3. Results
3.1. Histological Analysis and Cytogenetics
3.2. Analysis of Gene Expression of Amh and Foxl2a
3.3. Number of Gene Copies between Individuals with and without B Chromosomes
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Gene | Primer Sequence (5′–3′) | Use |
---|---|---|
ame_amh_Fw1 | CTGGGATGTTGAAGACGA | Conventional PCR |
ame_amh_Rv1 | GAGGAATTAATCAGCTCCAGAA | Conventional PCR |
ame_fox2_Fw1 | ACGTTCTTGGGCTCAGAGGA | Conventional PCR |
ame_foxl2a_Rv1 | AGACTTGCCGGGTTGGAAGTG | Conventional PCR |
amh_rtf1 | CCTCACTGCTCTTCCTGACG | qRT-PCR |
amh_rtR1 | AAACACCCAACACAGCTTGC | qRT-PCR |
foxl2a_rtF1 | ACCTGAGCCTTAACGAGTGC | qRT-PCR |
foxl2a_rtR1 | ATGTCTTCACACGTCGGGTC | qRT-PCR |
β-actin F_RT | ATCATGAAGTGCGACGTGGA | qRT-PCR |
β-actin R_RT | TATTTACGCTCAGGTGGGGC | qRT-PCR |
Gene | ♀ (B+) | ♀ (B−) | ♂ (B+) | ♂ (B−) | Total |
---|---|---|---|---|---|
amh | 3 | 3 | 3 | 3 | 12 |
foxl2a | 3 | 3 | 3 | 3 | 12 |
Total | 24 |
Sex | Samples | Number of B | Number of Reads (mi) | Coverage |
---|---|---|---|---|
Male | 2 | 0 | 93.7 | 7.5 |
1 | 1 | 52.2 | 4.2 | |
Female | 1 | 0 | 43.7 | 3.5 |
2 | 1 | 96 | 7.7 |
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Castro, J.P.; Hattori, R.S.; Yoshinaga, T.T.; Silva, D.M.Z.d.A.; Ruiz-Ruano, F.J.; Foresti, F.; Santos, M.H.; Almeida, M.C.d.; Moreira-Filho, O.; Artoni, R.F. Differential Expression of Genes Related to Sexual Determination Can Modify the Reproductive Cycle of Astyanax scabripinnis (Characiformes: Characidae) in B Chromosome Carrier Individuals. Genes 2019, 10, 909. https://doi.org/10.3390/genes10110909
Castro JP, Hattori RS, Yoshinaga TT, Silva DMZdA, Ruiz-Ruano FJ, Foresti F, Santos MH, Almeida MCd, Moreira-Filho O, Artoni RF. Differential Expression of Genes Related to Sexual Determination Can Modify the Reproductive Cycle of Astyanax scabripinnis (Characiformes: Characidae) in B Chromosome Carrier Individuals. Genes. 2019; 10(11):909. https://doi.org/10.3390/genes10110909
Chicago/Turabian StyleCastro, Jonathan Pena, Ricardo Shohei Hattori, Túlio Teruo Yoshinaga, Duílio Mazzoni Zerbinato de Andrade Silva, Francisco J. Ruiz-Ruano, Fausto Foresti, Mateus Henrique Santos, Mara Cristina de Almeida, Orlando Moreira-Filho, and Roberto Ferreira Artoni. 2019. "Differential Expression of Genes Related to Sexual Determination Can Modify the Reproductive Cycle of Astyanax scabripinnis (Characiformes: Characidae) in B Chromosome Carrier Individuals" Genes 10, no. 11: 909. https://doi.org/10.3390/genes10110909
APA StyleCastro, J. P., Hattori, R. S., Yoshinaga, T. T., Silva, D. M. Z. d. A., Ruiz-Ruano, F. J., Foresti, F., Santos, M. H., Almeida, M. C. d., Moreira-Filho, O., & Artoni, R. F. (2019). Differential Expression of Genes Related to Sexual Determination Can Modify the Reproductive Cycle of Astyanax scabripinnis (Characiformes: Characidae) in B Chromosome Carrier Individuals. Genes, 10(11), 909. https://doi.org/10.3390/genes10110909