Next Article in Journal
Massive Loss of Olfactory Receptors But Not Trace Amine-Associated Receptors in the World’s Deepest-Living Fish (Pseudoliparis swirei)
Previous Article in Journal
Genetically Determined Physical Activity and Its Association with Circulating Blood Cells
Previous Article in Special Issue
Transcriptome-Based SNP Discovery and Validation in the Hybrid Zone of the Neotropical Annual Fish Genus Austrolebias
Open AccessArticle

Differential Expression of Genes Related to Sexual Determination Can Modify the Reproductive Cycle of Astyanax scabripinnis (Characiformes: Characidae) in B Chromosome Carrier Individuals

1
Departamento de Genética e Evolução, Programa de Pós-Graduação em Biologia Evolutiva e Genética Molecular, Universidade Federal de São Carlos, Rodovia Washington Luis, Km 235, Monjolinho, São Carlos, SP 13565-905, Brazil
2
Estação Experimental de Salmonicultura de Campos do Jordão, UPD-CJ (APTA/SAA), Campos do Jordão, São Paulo, SP 12460-000, Brazil
3
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo, Departamento de Cirurgia, Universidade de São Paulo, Butantã, Rua Professor Orlando Marque Paiva, São Paulo, SP 05508-270, Brazil
4
Departamento de Morfologia, Instituto de Biociências, Universidade Estadual Paulista, Distrito de Rubião Junior, s/n, Botucatu, SP 18618-970, Brazil
5
Department of Ecology and Genetics, Evolutionary Biology Centre, Uppsala University, SE-752 36 Uppsala, Sweden
6
Departamento de Biologia Estrutural, Molecular e Genética, Programa de Pós-Graduação em Biologia Evolutiva, Universidade Estadual de Ponta Grossa, Avenida Carlos Cavalcanti, 4748, Ponta Grossa, PR 84030-900, Brazil
*
Author to whom correspondence should be addressed.
Genes 2019, 10(11), 909; https://doi.org/10.3390/genes10110909
Received: 4 October 2019 / Revised: 1 November 2019 / Accepted: 4 November 2019 / Published: 8 November 2019
The species complex Astyanax scabripinnis is one of the most studied with respect to origin, distribution, and frequency of B chromosomes, and is considered a model organism for evolutionary studies. Research using population inferences about the occurrence and frequency of the B chromosome shows seasonal variation between sexes, which is associated with the presence of this supernumerary element. We hypothesized that the B chromosome could influence the sex ratio of these animals. Based on this assumption, the present work aimed to investigate if differences exist among levels of gene expression with qRT-PCR of the amh (associated with testicular differentiation) and foxl2a (associated with ovarian differentiation) genes between B-carrier and non-B-carrier individuals. The results showed that for the amh gene, the difference in expression between animals with B chromosomes was not accentuated compared to that in animals without this chromosome. Expression of foxl2a in B-carrier females, however, was reduced by 73.56% compared to females that lacked the B chromosome. Males had no difference in expression of the amh and foxl2a genes between carriers and non-carriers of the B chromosome. Results indicate that the presence of B chromosomes is correlated with the differential expression of sex-associated genes. An analysis of these results integrated with data from other studies on the reproductive cycle in the same species reveals that this difference in expression may be expanding the reproductive cycle of the species. View Full-Text
Keywords: fishes; supernumerary chromosomes; sexual differentiation; foxl2a; amh fishes; supernumerary chromosomes; sexual differentiation; foxl2a; amh
Show Figures

Figure 1

MDPI and ACS Style

Castro, J.P.; Hattori, R.S.; Yoshinaga, T.T.; Silva, D.M.Z.A.; Ruiz-Ruano, F.J.; Foresti, F.; Santos, M.H.; Almeida, M.C.; Moreira-Filho, O.; Artoni, R.F. Differential Expression of Genes Related to Sexual Determination Can Modify the Reproductive Cycle of Astyanax scabripinnis (Characiformes: Characidae) in B Chromosome Carrier Individuals. Genes 2019, 10, 909.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop