Morphological Characterization of Flower Buds Development and Related Gene Expression Profiling at Bud Break Stage in Heterodichogamous Cyclocarya paliurus (Batal.) lljinskaja
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant for Sample Collection
2.2. RNA Extraction, cDNA Synthesis, and Sequencing
2.3. Data Filtering, De Novo Assembly, and Functional Annotation
2.4. Differentially Expressed Genes Between Female and Male Buds in Two Mating Types
2.5. Quantitative Real-Time PCR Verification
3. Results
3.1. Morphological Characteristics of Floral Bud Development
3.2. Sequencing, Assembly, and Annotation
3.3. Expression of Major Genes Related to Flower Differentiation
3.4. Identification of Differentially Expressed Genes
3.5. KEGG Pathway Annotation of Differentially Expressed Genes
3.6. Major Differentially Expressed Transcription Factors
3.7. Validation of RNA-Seq Result by qRT-PCR
4. Discussion
4.1. Expression Pattern of Major Genes in Different Pathways of Forming Flowers
4.2. Expression Pattern of TFs Related to Flowering from DEGs
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Database | Count | Percentage (%) |
---|---|---|
BLASTP | 22,563 | 35.51 |
BLASTX | 26,158 | 41.17 |
GO | 25,167 | 39.61 |
KO | 11,027 | 17.36 |
NR | 37,375 | 58.83 |
NT | 21,777 | 34.28 |
PFAM | 23,464 | 36.93 |
Prot | 31,330 | 49.31 |
SignalP | 2154 | 3.39 |
COG | 11,394 | 17.93 |
TmHMM | 6488 | 10.21 |
eggNOG | 13,476 | 21.21 |
Total annotation | 46,009 | 72.42 |
Total unigenes | 63,533 | 100 |
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Chen, X.; Mao, X.; Huang, P.; Fang, S. Morphological Characterization of Flower Buds Development and Related Gene Expression Profiling at Bud Break Stage in Heterodichogamous Cyclocarya paliurus (Batal.) lljinskaja. Genes 2019, 10, 818. https://doi.org/10.3390/genes10100818
Chen X, Mao X, Huang P, Fang S. Morphological Characterization of Flower Buds Development and Related Gene Expression Profiling at Bud Break Stage in Heterodichogamous Cyclocarya paliurus (Batal.) lljinskaja. Genes. 2019; 10(10):818. https://doi.org/10.3390/genes10100818
Chicago/Turabian StyleChen, Xiaoling, Xia Mao, Peng Huang, and Shengzuo Fang. 2019. "Morphological Characterization of Flower Buds Development and Related Gene Expression Profiling at Bud Break Stage in Heterodichogamous Cyclocarya paliurus (Batal.) lljinskaja" Genes 10, no. 10: 818. https://doi.org/10.3390/genes10100818