Identification and Comparison of Hyperglycemia-Induced Extracellular Vesicle Transcriptome in Different Mouse Stem Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals, Cell Isolation, and Culture
2.2. EV Isolation and Characterization
2.3. Cell/EV Lysate Preparation and Western Blotting
2.4. Nanoparticle Tracking Analysis (NTA)
2.5. RNA Sequencing and Generation of RNA Sequencing Datasets
2.6. RNAseq Data Analysis
2.7. Statistical Analysis
3. Results
3.1. Characterization of Stem/Progenitor Cell-Derived EVs
3.2. Hyperglycemia Significantly Alters Transcriptome in EPC-, CBSC-, and CPC-Derived EVs
3.3. Gene Analysis of EPC-, CBSC-, and CPC-Derived EVs Under Hyperglycemic Conditions
3.4. GO Analysis and Networking of Common mRNAs in EPC-, CBSC-, and CPC-Derived EVs
3.5. Common miRNAs and Basic Information of LncRNAs in EPC-, CBSC-, and CPC-Derived EVs
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Gene Name | Chromosome | Length | Strand | Exon | Start | End |
---|---|---|---|---|---|---|
Snhg5 | Chr9 | 1010 | − | 5 | 88521052 | 88522897 |
C430049B03Rik | ChrX | 2993 | − | 4 | 53053111 | 53057190 |
Rnu11 | Chr4 | 134 | − | 1 | 132270079 | 132270186 |
Rpph1 | Chr14 | 325 | − | 1 | 50807446 | 50807771 |
4933421O10Rik | Chr4 | 3850 | − | 2 | 33027138 | 33031323 |
Gm5478 | Chr15 | 2960 | − | - | 101643020 | 101647380 |
BC065397 | ChrX | 3243 | + | 10 | 136741824 | 136803364 |
A430093F15Rik | Chr19 | 2115 | + | 4 | 10740946 | 10786043 |
Cnot2 | Chr7 | 553 | − | 4 | 59556901 | 59587385 |
Gm14827 | ChrX | 3089 | + | 4 | 94442731 | 94447727 |
2310065F04Rik | Chr11 | 2724 | − | 5 | 67112460 | 67120080 |
Pfkfb2 | Chr13 | 383 | − | 3 | 47421808 | 47423198 |
Gm10814 | Chr19 | 1966 | + | 5 | 6012619 | 6018459 |
Prmt1 (H) | Chr4 | 1402 | − | 1 | 147615843 | 147617245 |
Gm10440 | Chr5 | 2753 | + | 4 | 54349990 | 54358543 |
5031426D15Rik | Chr2 | 6021 | − | 1 | 6922701 | 6928722 |
Gm15997 | Chr5 | 1491 | − | 5 | 149489055 | 149538030 |
Adh6−ps1 | Chr3 | - | + | 4 | 138374121 | 138388291 |
Hoxd11 | Chr2 | - | + | - | 74679558 | 74687016 |
1700011J10Rik | Chr2 | 1147 | + | - | 72979432 | 72989249 |
1700110C19Rik | Chr17 | 722 | + | 3 | 10324601 | 10329312 |
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Huang, G.; Garikipati, V.N.S.; Zhou, Y.; Benedict, C.; Houser, S.R.; Koch, W.J.; Kishore, R. Identification and Comparison of Hyperglycemia-Induced Extracellular Vesicle Transcriptome in Different Mouse Stem Cells. Cells 2020, 9, 2098. https://doi.org/10.3390/cells9092098
Huang G, Garikipati VNS, Zhou Y, Benedict C, Houser SR, Koch WJ, Kishore R. Identification and Comparison of Hyperglycemia-Induced Extracellular Vesicle Transcriptome in Different Mouse Stem Cells. Cells. 2020; 9(9):2098. https://doi.org/10.3390/cells9092098
Chicago/Turabian StyleHuang, Grace, Venkata Naga Srikanth Garikipati, Yan Zhou, Cynthia Benedict, Steven R. Houser, Walter J. Koch, and Raj Kishore. 2020. "Identification and Comparison of Hyperglycemia-Induced Extracellular Vesicle Transcriptome in Different Mouse Stem Cells" Cells 9, no. 9: 2098. https://doi.org/10.3390/cells9092098