Formation of βTC3 and MIN6 Pseudoislets Changes the Expression Pattern of Gpr40, Gpr55, and Gpr119 Receptors and Improves Lysophosphatidylcholines-Potentiated Glucose-Stimulated Insulin Secretion
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Cell Culture Conditions
2.2.1. βTC3 Cell Line
2.2.2. MIN6 Cell Line
2.2.3. MS1 Cell Line
2.3. Pseudoislets Formation
2.4. Stimulation of Ins1 Expression in Adherent βTC3 and βTC3-MS1 Pseudoislets
2.5. Glucose-Stimulated Insulin Secretion (GSIS)
2.6. RNA Isolation and Quantitative Reverse Transcription PCR Analysis
2.7. Statistical Analysis
3. Results
3.1. Comparison of the Responsiveness of βTC3 and MIN6 Monolayers as Well as Pseudoislets to Glipizide
3.2. Comparison of Insulin Secretory Activity of βTC3 and MIN6 Monolayers in Response to LPC with Various Acyl Chain Lengths
3.3. Comparison of Insulin Secretory Activity of βTC3 and MIN6 Pseudoislets in Response to LPC with Various Acyl Chain Lengths
4. Discussion
Author Contributions
Funding
Conflicts of Interest
References
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Gene Symbol | Forward Primer | Reverse Primer | Accession No. |
---|---|---|---|
Ins1 | 5′-ACCTGGAGACCTTAATGGGCCAAA-3′ | 5′-ATGACCTGCTTGCTGATGGTCTCT-3′ | NM_008386.3 |
Actβ | 5′-AAGAGCTATGAGCTGCCTGA-3 | 5′-TACGGATGTCAACGTCACAC-3′ | NM_007393.5 |
Gpr40 | 5′-TCTGCCTGGGGCCCTATAAT-3′ | 5′-TCCAGGACCTGTTCCCAAGT-3′ | NM_194057.3 |
Gpr55 | 5′-AGCCTTCTGACTTGGACAGC-3′ | 5′-CCTCATCCCCTTCATACTGG-3′ | NM_001033290.2 |
Gpr119 | 5′-CTTCTACTGTGACATGCTCAAGATTG-3 | 5′-CCATGGCTCCTGCATGTTC-3 | NM_181751.2 |
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Drzazga, A.; Cichońska, E.; Koziołkiewicz, M.; Gendaszewska-Darmach, E. Formation of βTC3 and MIN6 Pseudoislets Changes the Expression Pattern of Gpr40, Gpr55, and Gpr119 Receptors and Improves Lysophosphatidylcholines-Potentiated Glucose-Stimulated Insulin Secretion. Cells 2020, 9, 2062. https://doi.org/10.3390/cells9092062
Drzazga A, Cichońska E, Koziołkiewicz M, Gendaszewska-Darmach E. Formation of βTC3 and MIN6 Pseudoislets Changes the Expression Pattern of Gpr40, Gpr55, and Gpr119 Receptors and Improves Lysophosphatidylcholines-Potentiated Glucose-Stimulated Insulin Secretion. Cells. 2020; 9(9):2062. https://doi.org/10.3390/cells9092062
Chicago/Turabian StyleDrzazga, Anna, Eliza Cichońska, Maria Koziołkiewicz, and Edyta Gendaszewska-Darmach. 2020. "Formation of βTC3 and MIN6 Pseudoislets Changes the Expression Pattern of Gpr40, Gpr55, and Gpr119 Receptors and Improves Lysophosphatidylcholines-Potentiated Glucose-Stimulated Insulin Secretion" Cells 9, no. 9: 2062. https://doi.org/10.3390/cells9092062