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Article

The WAVE Regulatory Complex Is Required to Balance Protrusion and Adhesion in Migration

1
CRUK Beatson Institute, Glasgow G61 1BD, UK
2
School of Chemistry and Bioscience, University of Bradford, Bradford BD7 1PD, UK
3
Department of Biochemistry and Cell Biology, Geisel School of Medicine at Dartmouth College, Hanover, NH 03755-3844, USA
4
Zoological Institute, Technische Universität Braunschweig, 38106 Braunschweig, Germany
5
Institute of Cancer Sciences, University of Glasgow, Glasgow G61 1QH, UK
*
Author to whom correspondence should be addressed.
Cells 2020, 9(7), 1635; https://doi.org/10.3390/cells9071635
Received: 31 May 2020 / Revised: 2 July 2020 / Accepted: 3 July 2020 / Published: 7 July 2020
(This article belongs to the Special Issue Actin-Myosin Cytoskeleton Regulation and Function)
Cells migrating over 2D substrates are required to polymerise actin at the leading edge to form lamellipodia protrusions and nascent adhesions to anchor the protrusion to the substrate. The major actin nucleator in lamellipodia formation is the Arp2/3 complex, which is activated by the WAVE regulatory complex (WRC). Using inducible Nckap1 floxed mouse embryonic fibroblasts (MEFs), we confirm that the WRC is required for lamellipodia formation, and importantly, for generating the retrograde flow of actin from the leading cell edge. The loss of NCKAP1 also affects cell spreading and focal adhesion dynamics. In the absence of lamellipodium, cells can become elongated and move with a single thin pseudopod, which appears devoid of N-WASP. This phenotype was more prevalent on collagen than fibronectin, where we observed an increase in migratory speed. Thus, 2D cell migration on collagen is less dependent on branched actin. View Full-Text
Keywords: actin cytoskeleton; WAVE complex; stress fibers; focal adhesions; migration actin cytoskeleton; WAVE complex; stress fibers; focal adhesions; migration
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MDPI and ACS Style

Whitelaw, J.A.; Swaminathan, K.; Kage, F.; Machesky, L.M. The WAVE Regulatory Complex Is Required to Balance Protrusion and Adhesion in Migration. Cells 2020, 9, 1635. https://doi.org/10.3390/cells9071635

AMA Style

Whitelaw JA, Swaminathan K, Kage F, Machesky LM. The WAVE Regulatory Complex Is Required to Balance Protrusion and Adhesion in Migration. Cells. 2020; 9(7):1635. https://doi.org/10.3390/cells9071635

Chicago/Turabian Style

Whitelaw, Jamie A., Karthic Swaminathan, Frieda Kage, and Laura M. Machesky. 2020. "The WAVE Regulatory Complex Is Required to Balance Protrusion and Adhesion in Migration" Cells 9, no. 7: 1635. https://doi.org/10.3390/cells9071635

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