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Open AccessArticle

Heparin Anticoagulant for Human Bone Marrow Does Not Influence In Vitro Performance of Human Mesenchymal Stromal Cells

1
Department of Orthopedic Surgery, Graded Implants and Regenerative Strategies, OE 8893, Hannover Medical School (MHH), 30625 Hannover, Germany
2
Lower Saxony Centre for Biomedical Engineering, Implant Research and Development (NIFE), 30625 Hannover, Germany
3
Research Core Unit Genomics, OE 9415, Hannover Medical School (MHH), Hannover, Germany
4
Department of Orthopedic Surgery (Annastift), OE 6270, Hannover Medical School (MHH), 30625 Hannover, Germany
*
Author to whom correspondence should be addressed.
Joint last authors.
Cells 2020, 9(7), 1580; https://doi.org/10.3390/cells9071580
Received: 7 April 2020 / Revised: 18 June 2020 / Accepted: 25 June 2020 / Published: 29 June 2020
(This article belongs to the Section Stem Cells)
Mesenchymal stromal cells (MSCs) are a promising cell source for tissue engineering and regenerative medicine. In our lab, we found that MSC preparations from bone marrow of many different donors had a limited capacity of in vitro differentiation into osteogenic and chondrogenic lineages—a capacity claimed to be inherent to MSCs. The current study was designed to test the hypothesis that the amount of heparin used as anticoagulant during bone marrow harvest had an inhibitory influence on the in vitro differentiation capacity of isolated MSCs. Bone marrow was obtained from the femoral cavity of twelve donors during total hip arthroplasty in the absence or presence of heparin. No coagulation was observed in the absence of heparin. The number of mononuclear cells was independent of heparin addition. Isolated MSCs were characterized by morphology, population doubling times, expression of cell surface antigens and in vitro differentiation. Results of these analyses were independent of the amount of heparin. Transcriptome analyses of cells from three randomly chosen donors and quantitative realtime PCR (qRT-PCR) analysis from cells of all donors demonstrated no clear effect of heparin on the transcriptome of the cells. This excludes heparin as a potential source of disparate results.
Keywords: unfractionated heparin; ASA status; density gradient; cell heterogeneity; osteogenic differentiation; Alizarin Red S stain; marker genes; transcriptome analyses unfractionated heparin; ASA status; density gradient; cell heterogeneity; osteogenic differentiation; Alizarin Red S stain; marker genes; transcriptome analyses
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MDPI and ACS Style

Roger, Y.; Burmeister, L.; Hamm, A.; Elger, K.; Dittrich-Breiholz, O.; Flörkemeier, T.; Hoffmann, A. Heparin Anticoagulant for Human Bone Marrow Does Not Influence In Vitro Performance of Human Mesenchymal Stromal Cells. Cells 2020, 9, 1580.

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