Mistletoe-Extract Drugs Stimulate Anti-Cancer Vγ9Vδ2 T Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Collection
2.2. Cell Culture and Treatments
2.3. Flow Cytometry and Antibody Reagents
2.4. Cell Sorting, RNA Isolation, and CDR3 Analysis
2.5. Data Availability
2.6. Statistics
3. Results
3.1. AbnobaViscum but Not Iscador Mistletoe Extracts Induce Specific Expansion of Vγ9Vδ2 T Cells
3.2. AbnobaViscum Rapidly Stimulates the Release of Cytotoxic Granules and the Production of IFNγ and TNFα in Vγ9Vδ2 T Cells
3.3. AbnobaViscum Stimulation of Vγ9Vδ2 T Cells is Direct and BTN3A-Dependent
3.4. The AbnobaViscum-Responsive Vγ9Vδ2 TCR Repertoire is Similar to the Phosphoantigen-Responsive Repertoire
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Ma, L.; Phalke, S.; Stévigny, C.; Souard, F.; Vermijlen, D. Mistletoe-Extract Drugs Stimulate Anti-Cancer Vγ9Vδ2 T Cells. Cells 2020, 9, 1560. https://doi.org/10.3390/cells9061560
Ma L, Phalke S, Stévigny C, Souard F, Vermijlen D. Mistletoe-Extract Drugs Stimulate Anti-Cancer Vγ9Vδ2 T Cells. Cells. 2020; 9(6):1560. https://doi.org/10.3390/cells9061560
Chicago/Turabian StyleMa, Ling, Swati Phalke, Caroline Stévigny, Florence Souard, and David Vermijlen. 2020. "Mistletoe-Extract Drugs Stimulate Anti-Cancer Vγ9Vδ2 T Cells" Cells 9, no. 6: 1560. https://doi.org/10.3390/cells9061560