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Open AccessArticle

Extracellular Vesicles from Skeletal Muscle Cells Efficiently Promote Myogenesis in Induced Pluripotent Stem Cells

1
Institute of Biochemistry and Cell Biology, National Research Council, 00015 Rome, Italy
2
Department of Biotechnology and Life Sciences, University of Insubria, 21100 Varese, Italy
3
Gemelli Molise Hospital, 86100 Campobasso, Italy
4
Flow Cytometry Core, Humanitas Clinical and Research Center, 20089 Milan, Italy
5
Institute for Endocrinology and Oncology “Gaetano Salvatore”, National Research Council, 80131 Naples, Italy
6
IRCCS MultiMedica, 20138 Milan, Italy
7
Department of Biology, University of Rome Tor Vergata, 00133 Rome, Italy
8
Institute of Biomedical Technologies, National Research Council, 20090 Milan, Italy
9
Fondazione Istituto Nazionale di Genetica Molecolare, 20122 Milan, Italy
*
Authors to whom correspondence should be addressed.
Cells 2020, 9(6), 1527; https://doi.org/10.3390/cells9061527
Received: 19 April 2020 / Revised: 13 June 2020 / Accepted: 15 June 2020 / Published: 23 June 2020
The recent advances, offered by cell therapy in the regenerative medicine field, offer a revolutionary potential for the development of innovative cures to restore compromised physiological functions or organs. Adult myogenic precursors, such as myoblasts or satellite cells, possess a marked regenerative capacity, but the exploitation of this potential still encounters significant challenges in clinical application, due to low rate of proliferation in vitro, as well as a reduced self-renewal capacity. In this scenario, induced pluripotent stem cells (iPSCs) can offer not only an inexhaustible source of cells for regenerative therapeutic approaches, but also a valuable alternative for in vitro modeling of patient-specific diseases. In this study we established a reliable protocol to induce the myogenic differentiation of iPSCs, generated from pericytes and fibroblasts, exploiting skeletal muscle-derived extracellular vesicles (EVs), in combination with chemically defined factors. This genetic integration-free approach generates functional skeletal myotubes maintaining the engraftment ability in vivo. Our results demonstrate evidence that EVs can act as biological “shuttles” to deliver specific bioactive molecules for a successful transgene-free differentiation offering new opportunities for disease modeling and regenerative approaches. View Full-Text
Keywords: iPSC; extracellular vesicles; pericytes; skeletal muscle iPSC; extracellular vesicles; pericytes; skeletal muscle
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MDPI and ACS Style

Baci, D.; Chirivì, M.; Pace, V.; Maiullari, F.; Milan, M.; Rampin, A.; Somma, P.; Presutti, D.; Garavelli, S.; Bruno, A.; Cannata, S.; Lanzuolo, C.; Gargioli, C.; Rizzi, R.; Bearzi, C. Extracellular Vesicles from Skeletal Muscle Cells Efficiently Promote Myogenesis in Induced Pluripotent Stem Cells. Cells 2020, 9, 1527.

AMA Style

Baci D, Chirivì M, Pace V, Maiullari F, Milan M, Rampin A, Somma P, Presutti D, Garavelli S, Bruno A, Cannata S, Lanzuolo C, Gargioli C, Rizzi R, Bearzi C. Extracellular Vesicles from Skeletal Muscle Cells Efficiently Promote Myogenesis in Induced Pluripotent Stem Cells. Cells. 2020; 9(6):1527.

Chicago/Turabian Style

Baci, Denisa; Chirivì, Maila; Pace, Valentina; Maiullari, Fabio; Milan, Marika; Rampin, Andrea; Somma, Paolo; Presutti, Dario; Garavelli, Silvia; Bruno, Antonino; Cannata, Stefano; Lanzuolo, Chiara; Gargioli, Cesare; Rizzi, Roberto; Bearzi, Claudia. 2020. "Extracellular Vesicles from Skeletal Muscle Cells Efficiently Promote Myogenesis in Induced Pluripotent Stem Cells" Cells 9, no. 6: 1527.

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