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Unilateral Cleavage Furrows in Multinucleate Cells

Max Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany
LMU Munich, Department of Cell Biology (Anatomy III), Biomedical Center, D-82152 Planegg-Martinsried, Germany
Author to whom correspondence should be addressed.
Cells 2020, 9(6), 1493;
Received: 28 May 2020 / Revised: 15 June 2020 / Accepted: 16 June 2020 / Published: 18 June 2020
(This article belongs to the Special Issue Symmetry Breaking in Cells and Tissues)
Multinucleate cells can be produced in Dictyostelium by electric pulse-induced fusion. In these cells, unilateral cleavage furrows are formed at spaces between areas that are controlled by aster microtubules. A peculiarity of unilateral cleavage furrows is their propensity to join laterally with other furrows into rings to form constrictions. This means cytokinesis is biphasic in multinucleate cells, the final abscission of daughter cells being independent of the initial direction of furrow progression. Myosin-II and the actin filament cross-linking protein cortexillin accumulate in unilateral furrows, as they do in the normal cleavage furrows of mononucleate cells. In a myosin-II-null background, multinucleate or mononucleate cells were produced by cultivation either in suspension or on an adhesive substrate. Myosin-II is not essential for cytokinesis either in mononucleate or in multinucleate cells but stabilizes and confines the position of the cleavage furrows. In fused wild-type cells, unilateral furrows ingress with an average velocity of 1.7 µm × min−1, with no appreciable decrease of velocity in the course of ingression. In multinucleate myosin-II-null cells, some of the furrows stop growing, thus leaving space for the extensive broadening of the few remaining furrows. View Full-Text
Keywords: cell fusion; cortexillin; cytokinesis; Dictyostelium; myosin cell fusion; cortexillin; cytokinesis; Dictyostelium; myosin
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MDPI and ACS Style

Bindl, J.; Molnar, E.S.; Ecke, M.; Prassler, J.; Müller-Taubenberger, A.; Gerisch, G. Unilateral Cleavage Furrows in Multinucleate Cells. Cells 2020, 9, 1493.

AMA Style

Bindl J, Molnar ES, Ecke M, Prassler J, Müller-Taubenberger A, Gerisch G. Unilateral Cleavage Furrows in Multinucleate Cells. Cells. 2020; 9(6):1493.

Chicago/Turabian Style

Bindl, Julia, Eszter Sarolta Molnar, Mary Ecke, Jana Prassler, Annette Müller-Taubenberger, and Günther Gerisch. 2020. "Unilateral Cleavage Furrows in Multinucleate Cells" Cells 9, no. 6: 1493.

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