Emerin Phosphorylation during the Early Phase of the Oxidative Stress Response Influences Emerin–BAF Interaction and BAF Nuclear Localization
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Cultures, Transfection and Treatments
2.2. Western Blotting and Immunoprecipitation
2.3. Immunofluorescence and Proximity Ligation Assay
2.4. Antibodies
3. Results
3.1. ROS Generating Agents Affect Emerin Molecular Weight
3.2. Emerin Phosphorylation Increase Molecular Weight during the Early Phase of the Oxidative Stress Response
3.3. Emerin Interaction with BAF Is Affected during the Oxidative Stress Response
3.4. EDMD1 Cells Are More Sensitive to ROS-Induced DNA Damage
3.5. Emerin Is Also Affected in Various LaminA Deficiency Conditions
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Cenni, V.; Squarzoni, S.; Loi, M.; Mattioli, E.; Lattanzi, G.; Capanni, C. Emerin Phosphorylation during the Early Phase of the Oxidative Stress Response Influences Emerin–BAF Interaction and BAF Nuclear Localization. Cells 2020, 9, 1415. https://doi.org/10.3390/cells9061415
Cenni V, Squarzoni S, Loi M, Mattioli E, Lattanzi G, Capanni C. Emerin Phosphorylation during the Early Phase of the Oxidative Stress Response Influences Emerin–BAF Interaction and BAF Nuclear Localization. Cells. 2020; 9(6):1415. https://doi.org/10.3390/cells9061415
Chicago/Turabian StyleCenni, Vittoria, Stefano Squarzoni, Manuela Loi, Elisabetta Mattioli, Giovanna Lattanzi, and Cristina Capanni. 2020. "Emerin Phosphorylation during the Early Phase of the Oxidative Stress Response Influences Emerin–BAF Interaction and BAF Nuclear Localization" Cells 9, no. 6: 1415. https://doi.org/10.3390/cells9061415