Next Article in Journal
Crucial Role of Lamin A/C in the Migration and Differentiation of MSCs in Bone
Next Article in Special Issue
Hyaluronic Acid (HA) Receptors and the Motility of Schwann Cell(-Like) Phenotypes
Previous Article in Journal
Parallel Acquisition of Plasma Membrane Ultrastructure and Cytosolic Protein Localisation in Cultured Cells via Correlated Immunogold SEM
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Cells
Volume 9
Issue 6
10.3390/cells9061328
cells-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Anti-Inflammatory Performance of Lactose-Modified Chitosan and Hyaluronic Acid Mixtures in an In Vitro Macrophage-Mediated Inflammation Osteoarthritis Model

by
Elena Tarricone
1,†,
Elena Mattiuzzo
1,†,
Elisa Belluzzi
2,
Rossella Elia
1,
Andrea Benetti
1,
Rina Venerando
3,
Vincenzo Vindigni
4,
Pietro Ruggieri
5 and
Paola Brun
1,*
1
Department of Molecular Medicine, Histology Unit, University of Padova, 35121 Padova, Italy
2
Musculoskeletal Pathology and Oncology Laboratory, Orthopedic Clinic, Department of Surgery, Oncology and Gastroenterology, University of Padova, 35128 Padova, Italy
3
Department of Molecular Medicine, University of Padova, 35121 Padova, Italy
4
Clinic of Plastic and Reconstructive Surgery, University of Padova, 35128 Padova, Italy
5
Orthopedic Clinic, Department of Surgery, Oncology and Gastroenterology, University of Padova, 35128 Padova, Italy
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Cells 2020, 9(6), 1328; https://doi.org/10.3390/cells9061328
Submission received: 3 April 2020 / Revised: 17 May 2020 / Accepted: 19 May 2020 / Published: 26 May 2020
(This article belongs to the Special Issue Hyaluronic Acid: Basic and Clinical Aspects)
Browse Figures
Versions Notes

Abstract

The development and progression of osteoarthritis (OA) is associated with macrophage-mediated inflammation that generates a broad spectrum of cytokines and reactive oxygen species (ROS). This study investigates the effects of mid-MW hyaluronic acid (HA) in combination with a lactose-modified chitosan (CTL), on pro-inflammatory molecules and metalloproteinases (MMPs) expression, using an in vitro model of macrophage-mediated inflammation. Methods. To assess chondrocyte response to HA and CTL in the presence of macrophage derived inflammatory mediators, cells were exposed to the conditioned medium (CM) of U937 activated monocytes and changes in cell viability, pro-inflammatory mediators and MMPs expression or ROS generation were analysed. Results. CTL induced changes in chondrocyte viability that are reduced by the presence of HA. The CM of activated U937 monocytes (macrophages) significantly increased gene expression of pro-inflammatory molecules and MMPs and intracellular ROS generation in human chondrocyte cultures. HA, CTL and their combinations counteracted the oxidative damage and restored gene transcription for IL-1β, TNF-α, Gal-1, MMP-3 and MMP-13 to near baseline values. Conclusions. This study suggests that HA-CTL mixture attenuated macrophage-induced inflammation, inhibited MMPs expression and exhibited anti-oxidative effects. This evidence provides an initial step toward the development of an early stage OA therapeutic treatment
Keywords: chondrocyte; osteoarthritis; inflammation; chitosan; hyaluronic acid chondrocyte; osteoarthritis; inflammation; chitosan; hyaluronic acid

Share and Cite

MDPI and ACS Style

Tarricone, E.; Mattiuzzo, E.; Belluzzi, E.; Elia, R.; Benetti, A.; Venerando, R.; Vindigni, V.; Ruggieri, P.; Brun, P. Anti-Inflammatory Performance of Lactose-Modified Chitosan and Hyaluronic Acid Mixtures in an In Vitro Macrophage-Mediated Inflammation Osteoarthritis Model. Cells 2020, 9, 1328. https://doi.org/10.3390/cells9061328

AMA Style

Tarricone E, Mattiuzzo E, Belluzzi E, Elia R, Benetti A, Venerando R, Vindigni V, Ruggieri P, Brun P. Anti-Inflammatory Performance of Lactose-Modified Chitosan and Hyaluronic Acid Mixtures in an In Vitro Macrophage-Mediated Inflammation Osteoarthritis Model. Cells. 2020; 9(6):1328. https://doi.org/10.3390/cells9061328

Chicago/Turabian Style

Tarricone, Elena, Elena Mattiuzzo, Elisa Belluzzi, Rossella Elia, Andrea Benetti, Rina Venerando, Vincenzo Vindigni, Pietro Ruggieri, and Paola Brun. 2020. "Anti-Inflammatory Performance of Lactose-Modified Chitosan and Hyaluronic Acid Mixtures in an In Vitro Macrophage-Mediated Inflammation Osteoarthritis Model" Cells 9, no. 6: 1328. https://doi.org/10.3390/cells9061328

APA Style

Tarricone, E., Mattiuzzo, E., Belluzzi, E., Elia, R., Benetti, A., Venerando, R., Vindigni, V., Ruggieri, P., & Brun, P. (2020). Anti-Inflammatory Performance of Lactose-Modified Chitosan and Hyaluronic Acid Mixtures in an In Vitro Macrophage-Mediated Inflammation Osteoarthritis Model. Cells, 9(6), 1328. https://doi.org/10.3390/cells9061328

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop