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Article

The Essential Role of anxA2 in Langerhans Cell Birbeck Granules Formation

1
Department of Molecular Microbiology and Immunology, University of Southern California, Los Angeles, CA 90033, USA
2
Oak Crest Institute of Science, Monrovia, CA 91016, USA
3
Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, CA 90033, USA
4
Department of Obstetrics & Gynecology, University of Southern California, Los Angeles, CA 90033, USA
*
Author to whom correspondence should be addressed.
Cells 2020, 9(4), 974; https://doi.org/10.3390/cells9040974
Received: 5 March 2020 / Revised: 1 April 2020 / Accepted: 12 April 2020 / Published: 15 April 2020
(This article belongs to the Special Issue Recent Developments in Annexin Biology)
Langerhans cells (LC) are the resident antigen presenting cells of the mucosal epithelium and play an essential role in initiating immune responses. LC are the only cells in the body to contain Birbeck granules (BG), which are unique cytoplasmic organelles comprised of c-type lectin langerin. Studies of BG have historically focused on morphological characterizations, but BG have also been implicated in viral antigen processing which suggests that they can serve a function in antiviral immunity. This study focused on investigating proteins that could be involved in BG formation to further characterize their structure using transmission electron microscopy (TEM). Here, we report a critical role for the protein annexin A2 (anxA2) in the proper formation of BG structures. When anxA2 expression is downregulated, langerin expression decreases, cytoplasmic BG are nearly ablated, and the presence of malformed BG-like structures increases. Furthermore, in the absence of anxA2, we found langerin was no longer localized to BG or BG-like structures. Taken together, these results indicate an essential role for anxA2 in facilitating the proper formation of BG. View Full-Text
Keywords: anxA2; Birbeck granules; Langerhans cell; A2t anxA2; Birbeck granules; Langerhans cell; A2t
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MDPI and ACS Style

Thornton, S.M.; Samararatne, V.D.; Skeate, J.G.; Buser, C.; Lühen, K.P.; Taylor, J.R.; Da Silva, D.M.; Kast, W.M. The Essential Role of anxA2 in Langerhans Cell Birbeck Granules Formation. Cells 2020, 9, 974. https://doi.org/10.3390/cells9040974

AMA Style

Thornton SM, Samararatne VD, Skeate JG, Buser C, Lühen KP, Taylor JR, Da Silva DM, Kast WM. The Essential Role of anxA2 in Langerhans Cell Birbeck Granules Formation. Cells. 2020; 9(4):974. https://doi.org/10.3390/cells9040974

Chicago/Turabian Style

Thornton, Shantae M., Varsha D. Samararatne, Joseph G. Skeate, Christopher Buser, Kim P. Lühen, Julia R. Taylor, Diane M. Da Silva, and W. M. Kast 2020. "The Essential Role of anxA2 in Langerhans Cell Birbeck Granules Formation" Cells 9, no. 4: 974. https://doi.org/10.3390/cells9040974

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