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Open AccessArticle

Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture

1
NMI Natural and Medical Sciences Institute at the University of Tübingen, 72770 Reutlingen, Germany
2
Institute of Medical Immunology and BIH Center for Regenerative Therapies (BCRT), Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 13353 Berlin, Germany
3
Applied Chemistry, University of Reutlingen, 72762 Reutlingen, Germany
4
Department of Women’s Health, Research Institute for Women’s Health, Eberhard-Karls-University Tübingen, 72076 Tübingen, Germany
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Department of Cardiothoracic Surgery, Royal Brompton and Harefield Foundation Trust, Harefield Hospital Hill End Rd, Harefiled UB9 6JH, UK
6
Cluster of Excellence iFIT (EXC 2180) “Image-Guided and Functionally Instructed Tumor Therapies”, Eberhard-Karls-University Tübingen, 72076 Tübingen, Germany
7
Department of Medicine/Cardiology, Cardiovascular Research Laboratories, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA
*
Author to whom correspondence should be addressed.
Cells 2020, 9(3), 778; https://doi.org/10.3390/cells9030778
Received: 25 February 2020 / Revised: 15 March 2020 / Accepted: 19 March 2020 / Published: 23 March 2020
(This article belongs to the Special Issue Stem Cell Research on Cardiology)
Appropriate mechanical properties and fast endothelialization of synthetic grafts are key to ensure long-term functionality of implants. We used a newly developed biostable polyurethane elastomer (TPCU) to engineer electrospun vascular scaffolds with promising mechanical properties (E-modulus: 4.8 ± 0.6 MPa, burst pressure: 3326 ± 78 mmHg), which were biofunctionalized with fibronectin (FN) and decorin (DCN). Neither uncoated nor biofunctionalized TPCU scaffolds induced major adverse immune responses except for minor signs of polymorph nuclear cell activation. The in vivo endothelial progenitor cell homing potential of the biofunctionalized scaffolds was simulated in vitro by attracting endothelial colony-forming cells (ECFCs). Although DCN coating did attract ECFCs in combination with FN (FN + DCN), DCN-coated TPCU scaffolds showed a cell-repellent effect in the absence of FN. In a tissue-engineering approach, the electrospun and biofunctionalized tubular grafts were cultured with primary-isolated vascular endothelial cells in a custom-made bioreactor under dynamic conditions with the aim to engineer an advanced therapy medicinal product. Both FN and FN + DCN functionalization supported the formation of a confluent and functional endothelial layer. View Full-Text
Keywords: vascular graft; endothelialization; tissue engineering; decorin; fibronectin; electrospinning; endothelial progenitor cells; bioreactor; biostable polyurethane vascular graft; endothelialization; tissue engineering; decorin; fibronectin; electrospinning; endothelial progenitor cells; bioreactor; biostable polyurethane
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Daum, R.; Visser, D.; Wild, C.; Kutuzova, L.; Schneider, M.; Lorenz, G.; Weiss, M.; Hinderer, S.; Stock, U.A.; Seifert, M.; Schenke-Layland, K. Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture. Cells 2020, 9, 778.

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