A Signaling Crosstalk between BMP9 and HGF/c-Met Regulates Mouse Adult Liver Progenitor Cell Survival
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents and Antibodies
2.2. Cell Lines and Culture Conditions
2.3. Analysis of Cell Number
2.4. Measurement of Caspase-3-like Enzymatic Activity
2.5. Measurement of Apoptotic Index
2.6. Protein Isolation and Western Blot Analysis
2.7. RNA Isolation and Reverse Transcription–quantitative Polymerase Chain Reaction (RT–qPCR)
2.8. Gene Silencing by shRNA and siRNA
2.9. Transcriptional Reporter Assay
2.10. Statistical Analysis
3. Results
3.1. HGF/c-Met Signaling Inhibits BMP9-Triggered Apoptosis in Oval Cells while Potentiating SMAD Signaling
3.2. BMP9 and HGF/c-Met Signaling Crosstalk in Oval Cells is Dependent on ALK1/SMAD1 Signaling Axis
3.3. p38MAPK Activation Mediates BMP9-Induced Apoptosis in Oval Cells
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Addante, A.; Roncero, C.; Lazcanoiturburu, N.; Méndez, R.; Almalé, L.; García-Álvaro, M.; ten Dijke, P.; Fabregat, I.; Herrera, B.; Sánchez, A. A Signaling Crosstalk between BMP9 and HGF/c-Met Regulates Mouse Adult Liver Progenitor Cell Survival. Cells 2020, 9, 752. https://doi.org/10.3390/cells9030752
Addante A, Roncero C, Lazcanoiturburu N, Méndez R, Almalé L, García-Álvaro M, ten Dijke P, Fabregat I, Herrera B, Sánchez A. A Signaling Crosstalk between BMP9 and HGF/c-Met Regulates Mouse Adult Liver Progenitor Cell Survival. Cells. 2020; 9(3):752. https://doi.org/10.3390/cells9030752
Chicago/Turabian StyleAddante, Annalisa, Cesáreo Roncero, Nerea Lazcanoiturburu, Rebeca Méndez, Laura Almalé, María García-Álvaro, Peter ten Dijke, Isabel Fabregat, Blanca Herrera, and Aránzazu Sánchez. 2020. "A Signaling Crosstalk between BMP9 and HGF/c-Met Regulates Mouse Adult Liver Progenitor Cell Survival" Cells 9, no. 3: 752. https://doi.org/10.3390/cells9030752