Carbonic Anhydrase III Promotes Cell Migration and Epithelial–Mesenchymal Transition in Oral Squamous Cell Carcinoma
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. CA III Overexpressed System
2.3. RNA Interference Experiments
2.4. Migration and Invasion Assay
2.5. Reverse-Transcription PCR and Real-Time PCR
2.6. Western Blot
2.7. Gene Expression Microarray
2.8. Luciferase-Report Assay
2.9. Statistical Analyses
3. Results
3.1. Effect of CA III on Cell Growth, Motility, Migration, and Invasion in oral Cancer Cells
3.2. CA III Regulates EMT Markers in Oral Cancer Cells
3.3. CA III Inhibits the Promoter Activity of E-Cadherin and Promotes EMT-Related Transcription Factors Slug and Twist in Oral Cancer Cells
3.4. CA III Promotes the Migration Ability Through the FAK/Src Pathway in Oral Cancer Cells
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Chu, Y.-H.; Su, C.-W.; Hsieh, Y.-S.; Chen, P.-N.; Lin, C.-W.; Yang, S.-F. Carbonic Anhydrase III Promotes Cell Migration and Epithelial–Mesenchymal Transition in Oral Squamous Cell Carcinoma. Cells 2020, 9, 704. https://doi.org/10.3390/cells9030704
Chu Y-H, Su C-W, Hsieh Y-S, Chen P-N, Lin C-W, Yang S-F. Carbonic Anhydrase III Promotes Cell Migration and Epithelial–Mesenchymal Transition in Oral Squamous Cell Carcinoma. Cells. 2020; 9(3):704. https://doi.org/10.3390/cells9030704
Chicago/Turabian StyleChu, Yin-Hung, Chun-Wen Su, Yih-Shou Hsieh, Pei-Ni Chen, Chiao-Wen Lin, and Shun-Fa Yang. 2020. "Carbonic Anhydrase III Promotes Cell Migration and Epithelial–Mesenchymal Transition in Oral Squamous Cell Carcinoma" Cells 9, no. 3: 704. https://doi.org/10.3390/cells9030704