Next Article in Journal
Phase Diagram of Purified CNS Myelin Reveals Continuous Transformation between Expanded and Compacted Lamellar States
Next Article in Special Issue
Microautophagy in Plants: Consideration of Its Molecular Mechanism
Previous Article in Journal
Glucose Metabolic Reprogramming of ER Breast Cancer in Acquired Resistance to the CDK4/6 Inhibitor Palbociclib+
Previous Article in Special Issue
Autophagy Controls Sulphur Metabolism in the Rosette Leaves of Arabidopsis and Facilitates S Remobilization to the Seeds
Article

Overexpression of the Selective Autophagy Cargo Receptor NBR1 Modifies Plant Response to Sulfur Deficit

1
Department of Plant Biochemistry, Institute of Biochemistry and Biophysics Polish Academy of Sciences, Pawińskiego 5A St, 02-106 Warsaw, Poland
2
Laboratory of Mass Spectrometry, Institute of Biochemistry and Biophysics Polish Academy of Sciences, Pawińskiego 5A St, 02-106 Warsaw, Poland
*
Author to whom correspondence should be addressed.
Present address: MRC-University of Glasgow Center for Virus Research, 464 Bearsden Rd., Glasgow G61 1QH, Scotland, UK.
Cells 2020, 9(3), 669; https://doi.org/10.3390/cells9030669
Received: 10 January 2020 / Revised: 3 March 2020 / Accepted: 6 March 2020 / Published: 10 March 2020
(This article belongs to the Special Issue Advances in the Plant Autophagy)
Plants exposed to sulfur deficit elevate the transcription of NBR1 what might reflect an increased demand for NBR1 in such conditions. Therefore, we investigated the role of this selective autophagy cargo receptor in plant response to sulfur deficit (-S). Transcriptome analysis of the wild type and NBR1 overexpressing plants pointed out differences in gene expression in response to -S. Our attention focused particularly on the genes upregulated by -S in roots of both lines because of significant overrepresentation of cytoplasmic ribosomal gene family. Moreover, we noticed overrepresentation of the same family in the set of proteins co-purifying with NBR1 in -S. One of these ribosomal proteins, RPS6 was chosen for verification of its direct interaction with NBR1 and proven to bind outside the NBR1 ubiquitin binding domains. The biological significance of this novel interaction and the postulated role of NBR1 in ribosomes remodeling in response to starvation remain to be further investigated. Interestingly, NBR1 overexpressing seedlings have significantly shorter roots than wild type when grown in nutrient deficient conditions in the presence of TOR kinase inhibitors. This phenotype probably results from excessive autophagy induction by the additive effect of NBR1 overexpression, starvation, and TOR inhibition. View Full-Text
Keywords: autophagy; protein-protein interaction; sulfur deficit; transcriptome; Arabidopsis thaliana autophagy; protein-protein interaction; sulfur deficit; transcriptome; Arabidopsis thaliana
Show Figures

Figure 1

MDPI and ACS Style

Tarnowski, L.; Collados Rodriguez, M.; Brzywczy, J.; Cysewski, D.; Wawrzynska, A.; Sirko, A. Overexpression of the Selective Autophagy Cargo Receptor NBR1 Modifies Plant Response to Sulfur Deficit. Cells 2020, 9, 669. https://doi.org/10.3390/cells9030669

AMA Style

Tarnowski L, Collados Rodriguez M, Brzywczy J, Cysewski D, Wawrzynska A, Sirko A. Overexpression of the Selective Autophagy Cargo Receptor NBR1 Modifies Plant Response to Sulfur Deficit. Cells. 2020; 9(3):669. https://doi.org/10.3390/cells9030669

Chicago/Turabian Style

Tarnowski, Leszek, Milagros Collados Rodriguez, Jerzy Brzywczy, Dominik Cysewski, Anna Wawrzynska, and Agnieszka Sirko. 2020. "Overexpression of the Selective Autophagy Cargo Receptor NBR1 Modifies Plant Response to Sulfur Deficit" Cells 9, no. 3: 669. https://doi.org/10.3390/cells9030669

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop