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Flexible Players within the Sheaths: The Intrinsically Disordered Proteins of Myelin in Health and Disease
Article

Effect of Cholesterol and Myelin Basic Protein (MBP) Content on Lipid Monolayers Mimicking the Cytoplasmic Membrane of Myelin

1
Institut für Chemie, Martin-Luther-Universität Halle-Wittenberg, Von-Danckelmann-Platz 4, 06120 Halle (Saale), Germany
2
Interdisciplinary research center HALOmem at the Martin-Luther-Universität Halle-Wittenberg, 06120 Halle (Saale), Germany
3
Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON N1G 2W1, Canada
*
Author to whom correspondence should be addressed.
Cells 2020, 9(3), 529; https://doi.org/10.3390/cells9030529
Received: 11 January 2020 / Revised: 16 February 2020 / Accepted: 20 February 2020 / Published: 25 February 2020
(This article belongs to the Special Issue Structure and Function of Healthy and Diseased Myelin)
Myelin basic protein (MBP) is located in the insulating covers of nerve cells in the brain and spinal cord. By interacting with lipid membranes, it is responsible for compaction of the myelin sheath in the central nervous system, which is weakened in demyelinating diseases. The lipid composition of the myelin leaflet has a high impact on the interaction between the membrane and MBP. Cholesterol is present in the cytoplasmic leaflet with a rather high amount of 44% (mol%). In this study, the focus is on the effect of cholesterol, mainly by varying its content, on the interaction of MBP with a lipid monolayer. Therefore, Langmuir lipid monolayers mimicking the cytoplasmic membrane of myelin and monolayers with variations of cholesterol content between 0% and 100% were measured at the air/water interface with additional imaging by fluorescence microscopy. All experiments were performed with and without bovine MBP to study the dependence of the interaction of the protein with the monolayers on the cholesterol content. The native amount of 44% cholesterol in the monolayer combines optima in the order of the monolayer (presumably correlating to compaction and thermodynamic stability) and protein interaction and shows unique features in comparison to lower or higher cholesterol contents. View Full-Text
Keywords: intrinsically disordered proteins; myelin basic protein; multiple sclerosis; fluorescence microscopy; Langmuir monolayer; cholesterol intrinsically disordered proteins; myelin basic protein; multiple sclerosis; fluorescence microscopy; Langmuir monolayer; cholesterol
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MDPI and ACS Style

Träger, J.; Widder, K.; Kerth, A.; Harauz, G.; Hinderberger, D. Effect of Cholesterol and Myelin Basic Protein (MBP) Content on Lipid Monolayers Mimicking the Cytoplasmic Membrane of Myelin. Cells 2020, 9, 529. https://doi.org/10.3390/cells9030529

AMA Style

Träger J, Widder K, Kerth A, Harauz G, Hinderberger D. Effect of Cholesterol and Myelin Basic Protein (MBP) Content on Lipid Monolayers Mimicking the Cytoplasmic Membrane of Myelin. Cells. 2020; 9(3):529. https://doi.org/10.3390/cells9030529

Chicago/Turabian Style

Träger, Jennica, Katharina Widder, Andreas Kerth, George Harauz, and Dariush Hinderberger. 2020. "Effect of Cholesterol and Myelin Basic Protein (MBP) Content on Lipid Monolayers Mimicking the Cytoplasmic Membrane of Myelin" Cells 9, no. 3: 529. https://doi.org/10.3390/cells9030529

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