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Article

Transcriptional and Ultrastructural Analyses Suggest Novel Insights into Epithelial Barrier Impairment in Celiac Disease

1
Department of Pathology, The Children Memorial Health Institute, Al. Dzieci Polskich 20, 04-730 Warsaw, Poland
2
Department of Gastroenterology and Hepatology, University Hospital Zürich, CH-8001 Zürich, Switzerland
3
Clinic of Gastroenterology, The Children Memorial Health Institute, Al. Dzieci Polskich 20, 04-730 Warsaw, Poland
*
Author to whom correspondence should be addressed.
These authors contributed to the first authorship equally.
These authors contributed to the last authorship equally.
§
Present address: Faculty of Medicine and Health Sciences, Jan Kochanowski University, Al. IX Wieków Kielc 19A, 25-317 Kielce, Poland.
Cells 2020, 9(2), 516; https://doi.org/10.3390/cells9020516
Received: 13 January 2020 / Revised: 11 February 2020 / Accepted: 20 February 2020 / Published: 24 February 2020
(This article belongs to the Special Issue Epithelial Cell Mechanics: From Physiology to Pathology)
Disruption of epithelial junctional complex (EJC), especially tight junctions (TJ), resulting in increased intestinal permeability, is supposed to activate the enhanced immune response to gluten and to induce the development of celiac disease (CD). This study is aimed to present the role of EJC in CD pathogenesis. To analyze differentially expressed genes the next-generation mRNA sequencing data from CD326+ epithelial cells isolated from non-celiac and celiac patients were involved. Ultrastructural studies with morphometry of EJC were done in potential CD, newly recognized active CD, and non-celiac controls. The transcriptional analysis suggested disturbances of epithelium and the most significant gene ontology enriched terms in epithelial cells from CD patients related to the plasma membrane, extracellular exome, extracellular region, and extracellular space. Ultrastructural analyses showed significantly tighter TJ, anomalies in desmosomes, dilatations of intercellular space, and shorter microvilli in potential and active CD compared to controls. Enterocytes of fetal-like type and significantly wider adherence junctions were observed only in active CD. In conclusion, the results do not support the hypothesis that an increased passage of gluten peptides by unsealing TJ precedes CD development. However, increased intestinal permeability due to abnormality of epithelium might play a role in CD onset. View Full-Text
Keywords: celiac disease; epithelium; epithelial barrier; tight junctions; adherence junctions; fetal like type enterocytes celiac disease; epithelium; epithelial barrier; tight junctions; adherence junctions; fetal like type enterocytes
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MDPI and ACS Style

Sowińska, A.; Morsy, Y.; Czarnowska, E.; Oralewska, B.; Konopka, E.; Woynarowski, M.; Szymańska, S.; Ejmont, M.; Scharl, M.; Bierła, J.B.; Wawrzyniak, M.; Cukrowska, B. Transcriptional and Ultrastructural Analyses Suggest Novel Insights into Epithelial Barrier Impairment in Celiac Disease. Cells 2020, 9, 516. https://doi.org/10.3390/cells9020516

AMA Style

Sowińska A, Morsy Y, Czarnowska E, Oralewska B, Konopka E, Woynarowski M, Szymańska S, Ejmont M, Scharl M, Bierła JB, Wawrzyniak M, Cukrowska B. Transcriptional and Ultrastructural Analyses Suggest Novel Insights into Epithelial Barrier Impairment in Celiac Disease. Cells. 2020; 9(2):516. https://doi.org/10.3390/cells9020516

Chicago/Turabian Style

Sowińska, Agnieszka, Yasser Morsy, Elżbieta Czarnowska, Beata Oralewska, Ewa Konopka, Marek Woynarowski, Sylwia Szymańska, Maria Ejmont, Michael Scharl, Joanna B. Bierła, Marcin Wawrzyniak, and Bożena Cukrowska. 2020. "Transcriptional and Ultrastructural Analyses Suggest Novel Insights into Epithelial Barrier Impairment in Celiac Disease" Cells 9, no. 2: 516. https://doi.org/10.3390/cells9020516

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