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The Role of miR-21 in Osteoblasts–Osteoclasts Coupling In Vitro

Department of Experimental Biology, The Faculty of Biology and Animal Science, University of Environmental and Life Sciences, 50-375 Wroclaw, Poland
Laboratory of Gene Expression, Institute of Biotechnology CAS, Biocev, 25250 Vestec, Czech Republic
International Institute of Translational Medicine, Jesionowa 11 St, 55-124 Malin, Poland
Collegium Medicum, Cardinal Stefan Wyszyński University (UKSW), Woycickiego 1/3, 01-938 Warsaw, Poland
Author to whom correspondence should be addressed.
Cells 2020, 9(2), 479;
Received: 29 December 2019 / Revised: 17 February 2020 / Accepted: 18 February 2020 / Published: 19 February 2020
(This article belongs to the Collection Regulatory Functions of microRNAs)
MiR-21 is being gradually more and more recognized as a molecule regulating bone tissue homeostasis. However, its function is not fully understood due to the dual role of miR-21 on bone-forming and bone-resorbing cells. In this study, we investigated the impact of miR-21 inhibition on pre-osteoblastic cells differentiation and paracrine signaling towards pre-osteoclasts using indirect co-culture model of mouse pre-osteoblast (MC3T3) and pre-osteoclast (4B12) cell lines. The inhibition of miR-21 in MC3T3 cells (MC3T3inh21) modulated expression of genes encoding osteogenic markers including collagen type I (Coll-1), osteocalcin (Ocl), osteopontin (Opn), and runt-related transcription factor 2 (Runx-2). Inhibition of miR-21 in osteogenic cultures of MC3T3 also inflected the synthesis of OPN protein which is essential for proper mineralization of extracellular matrix (ECM) and anchoring osteoclasts to the bones. Furthermore, it was shown that in osteoblasts miR-21 regulates expression of factors that are vital for survival of pre-osteoclast, such as receptor activator of nuclear factor κB ligand (RANKL). The pre-osteoclast cultured with MC3T3inh21 cells was characterized by lowered expression of several markers associated with osteoclasts’ differentiation, foremost tartrate-resistant acid phosphatase (Trap) but also receptor activator of nuclear factor-κB ligand (Rank), cathepsin K (Ctsk), carbonic anhydrase II (CaII), and matrix metalloproteinase (Mmp-9). Collectively, our data indicate that the inhibition of miR-21 in MC3T3 cells impairs the differentiation and ECM mineralization as well as influences paracrine signaling leading to decreased viability of pre-osteoclasts. View Full-Text
Keywords: miR-21-5p; osteogenesis; differentiation; precursor cells; osteoblasts; osteoclasts miR-21-5p; osteogenesis; differentiation; precursor cells; osteoblasts; osteoclasts
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MDPI and ACS Style

Smieszek, A.; Marcinkowska, K.; Pielok, A.; Sikora, M.; Valihrach, L.; Marycz, K. The Role of miR-21 in Osteoblasts–Osteoclasts Coupling In Vitro. Cells 2020, 9, 479.

AMA Style

Smieszek A, Marcinkowska K, Pielok A, Sikora M, Valihrach L, Marycz K. The Role of miR-21 in Osteoblasts–Osteoclasts Coupling In Vitro. Cells. 2020; 9(2):479.

Chicago/Turabian Style

Smieszek, Agnieszka, Klaudia Marcinkowska, Ariadna Pielok, Mateusz Sikora, Lukas Valihrach, and Krzysztof Marycz. 2020. "The Role of miR-21 in Osteoblasts–Osteoclasts Coupling In Vitro" Cells 9, no. 2: 479.

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