Next Article in Journal
Future Therapeutic Directions for Smac-Mimetics
Previous Article in Journal
Mitochondria Associated Germinal Structures in Spermatogenesis: piRNA Pathway Regulation and Beyond
Previous Article in Special Issue
The Charcot–Marie Tooth Disease Mutation R94Q in MFN2 Decreases ATP Production but Increases Mitochondrial Respiration under Conditions of Mild Oxidative Stress
Open AccessArticle

Differential Sensitivity of Two Endothelial Cell Lines to Hydrogen Peroxide Toxicity: Relevance for In Vitro Studies of the Blood–Brain Barrier

1
Department of Medical Bioscience, University of the Western Cape, Bellville, Cape Town 7530, South Africa
2
Anatomy Department, Ladoke Akintola University of Technology, Ogbomoso 210241, Nigeria
*
Author to whom correspondence should be addressed.
Cells 2020, 9(2), 403; https://doi.org/10.3390/cells9020403 (registering DOI)
Received: 28 October 2019 / Revised: 20 December 2019 / Accepted: 6 January 2020 / Published: 10 February 2020
Oxidative stress (OS) has been linked to blood–brain barrier (BBB) dysfunction which in turn has been implicated in the initiation and propagation of some neurological diseases. In this study, we profiled, for the first time, two endothelioma cell lines of mouse brain origin, commonly used as in vitro models of the blood–brain barrier, for their resistance against oxidative stress using viability measures and glutathione contents as markers. OS was induced by exposing cultured cells to varying concentrations of hydrogen peroxide and fluorescence microscopy/spectrometry was used to detect and estimate cellular glutathione contents. A colorimetric viability assay was used to determine changes in the viability of OS-exposed cells. Both the b.End5 and bEnd.3 cell lines investigated showed demonstrable content of glutathione with a statistically insignificant difference in glutathione quantity per unit cell, but with a statistically significant higher capacity for the b.End5 cell line for de novo glutathione synthesis. Furthermore, the b.End5 cells demonstrated greater oxidant buffering capacity to higher concentrations of hydrogen peroxide than the bEnd.3 cells. We concluded that mouse brain endothelial cells, derived from different types of cell lines, differ enormously in their antioxidant characteristics. We hereby recommend caution in making comparisons across BBB models utilizing distinctly different cell lines and require further prerequisites to ensure that in vitro BBB models involving these cell lines are reliable and reproducible. View Full-Text
Keywords: oxidative stress; blood–brain barrier; bEnd5; bEnd.3; glutathione; viability oxidative stress; blood–brain barrier; bEnd5; bEnd.3; glutathione; viability
Show Figures

Figure 1

MDPI and ACS Style

Alamu, O.; Rado, M.; Ekpo, O.; Fisher, D. Differential Sensitivity of Two Endothelial Cell Lines to Hydrogen Peroxide Toxicity: Relevance for In Vitro Studies of the Blood–Brain Barrier. Cells 2020, 9, 403.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop