Mouse Embryonic Stem Cell-Derived Ureteric Bud Progenitors Induce Nephrogenesis
1
Center for Cell Matrix Research, Faculty of Biochemistry and Molecular Medicine, Biocenter Oulu, Laboratory of Developmental Biology, Infotech Oulu, University of Oulu, Aapistie 5A, 90220 Oulu, Finland
2
Borealis Biobank of Northern Finland, Oulu Central Hospital, 90220 Oulu, Finland
*
Authors to whom correspondence should be addressed.
Cells 2020, 9(2), 329; https://doi.org/10.3390/cells9020329
Received: 18 December 2019
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Revised: 16 January 2020
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Accepted: 27 January 2020
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Published: 31 January 2020
(This article belongs to the Special Issue 3D Stem Cell Culture)
Generation of kidney organoids from pluripotent stem cells (PSCs) is regarded as a potentially powerful way to study kidney development, disease, and regeneration. Direct differentiation of PSCs towards renal lineages is well studied; however, most of the studies relate to generation of nephron progenitor population from PSCs. Until now, differentiation of PSCs into ureteric bud (UB) progenitor cells has had limited success. Here, we describe a simple, efficient, and reproducible protocol to direct differentiation of mouse embryonic stem cells (mESCs) into UB progenitor cells. The mESC-derived UB cells were able to induce nephrogenesis when co-cultured with primary metanephric mesenchyme (pMM). In generated kidney organoids, the embryonic pMM developed nephron structures, and the mESC-derived UB cells formed numerous collecting ducts connected with the nephron tubules. Altogether, our study established an uncomplicated and reproducible platform to generate ureteric bud progenitors from mouse embryonic stem cells.
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Keywords:
mouse embryonic stem cell; differentiation protocol; ureteric bud progenitor cells; 3D kidney organoids
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MDPI and ACS Style
Tan, Z.; Rak-Raszewska, A.; Skovorodkin, I.; Vainio, S.J. Mouse Embryonic Stem Cell-Derived Ureteric Bud Progenitors Induce Nephrogenesis. Cells 2020, 9, 329. https://doi.org/10.3390/cells9020329
AMA Style
Tan Z, Rak-Raszewska A, Skovorodkin I, Vainio SJ. Mouse Embryonic Stem Cell-Derived Ureteric Bud Progenitors Induce Nephrogenesis. Cells. 2020; 9(2):329. https://doi.org/10.3390/cells9020329
Chicago/Turabian StyleTan, Zenglai, Aleksandra Rak-Raszewska, Ilya Skovorodkin, and Seppo J. Vainio. 2020. "Mouse Embryonic Stem Cell-Derived Ureteric Bud Progenitors Induce Nephrogenesis" Cells 9, no. 2: 329. https://doi.org/10.3390/cells9020329
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