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Open AccessFeature PaperArticle

Effect of a Lens Protein in Low-Temperature Culture of Novel Immortalized Human Lens Epithelial Cells (iHLEC-NY2)

1
Department of Ophthalmology, Kanazawa Medical University, Ishikawa 920-0293, Japan
2
Research Promotion and Support Headquarters, Fujita Health University, Aichi 470-1192, Japan
3
Faculty of Pharmacy, Kindai University, Osaka 577-8502, Japan
4
Division of Hygienic Chemistry, Faculty of Pharmacy, Keio University, Tokyo 105-8512, Japan
5
Radiation Biochemistry, Division of Radiation Life Science, Institute for Integrated Radiation and Nuclear Science, Kyoto University, Osaka 590-0494, Japan
6
Department of Electrical and Mechanical Engineering, Nagoya Institute of Technology, Aichi 466-8555, Japan
7
Center of Biomedical Physics and Information Technology, Nagoya Institute of Technology, Aichi 466-8555, Japan
*
Author to whom correspondence should be addressed.
Cells 2020, 9(12), 2670; https://doi.org/10.3390/cells9122670
Received: 4 November 2020 / Revised: 27 November 2020 / Accepted: 11 December 2020 / Published: 11 December 2020
The prevalence of nuclear cataracts was observed to be significantly higher among residents of tropical and subtropical regions compared to those of temperate and subarctic regions. We hypothesized that elevated environmental temperatures may pose a risk of nuclear cataract development. The results of our in silico simulation revealed that in temperate and tropical regions, the human lens temperature ranges from 35.0 °C to 37.5 °C depending on the environmental temperature. The medium temperature changes during the replacement regularly in the cell culture experiment were carefully monitored using a sensor connected to a thermometer and showed a decrease of 1.9 °C, 3.0 °C, 1.7 °C, and 0.1 °C, after 5 min when setting the temperature of the heat plate device at 35.0 °C, 37.5 °C, 40.0 °C, and 42.5 °C, respectively. In the newly created immortalized human lens epithelial cell line clone NY2 (iHLEC-NY2), the amounts of RNA synthesis of αA crystallin, protein expression, and amyloid β (Aβ)1-40 secreted into the medium were increased at the culture temperature of 37.5 °C compared to 35.0 °C. In short-term culture experiments, the secretion of Aβ1-40 observed in cataracts was increased at 37.5 °C compared to 35.0 °C, suggesting that the long-term exposure to a high-temperature environment may increase the risk of cataracts. View Full-Text
Keywords: immortalized human lens epithelial cell clone NY2; iHLEC-NY2; low-temperature culture; computer simulation; environmental temperature; crystalline lens temperature; αA crystallin; amyloid β immortalized human lens epithelial cell clone NY2; iHLEC-NY2; low-temperature culture; computer simulation; environmental temperature; crystalline lens temperature; αA crystallin; amyloid β
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MDPI and ACS Style

Yamamoto, N.; Takeda, S.; Hatsusaka, N.; Hiramatsu, N.; Nagai, N.; Deguchi, S.; Nakazawa, Y.; Takata, T.; Kodera, S.; Hirata, A.; Kubo, E.; Sasaki, H. Effect of a Lens Protein in Low-Temperature Culture of Novel Immortalized Human Lens Epithelial Cells (iHLEC-NY2). Cells 2020, 9, 2670. https://doi.org/10.3390/cells9122670

AMA Style

Yamamoto N, Takeda S, Hatsusaka N, Hiramatsu N, Nagai N, Deguchi S, Nakazawa Y, Takata T, Kodera S, Hirata A, Kubo E, Sasaki H. Effect of a Lens Protein in Low-Temperature Culture of Novel Immortalized Human Lens Epithelial Cells (iHLEC-NY2). Cells. 2020; 9(12):2670. https://doi.org/10.3390/cells9122670

Chicago/Turabian Style

Yamamoto, Naoki; Takeda, Shun; Hatsusaka, Natsuko; Hiramatsu, Noriko; Nagai, Noriaki; Deguchi, Saori; Nakazawa, Yosuke; Takata, Takumi; Kodera, Sachiko; Hirata, Akimasa; Kubo, Eri; Sasaki, Hiroshi. 2020. "Effect of a Lens Protein in Low-Temperature Culture of Novel Immortalized Human Lens Epithelial Cells (iHLEC-NY2)" Cells 9, no. 12: 2670. https://doi.org/10.3390/cells9122670

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