Cytokines TNFα, IFNγ and IL-2 Are Responsible for Signal Transmission from the Innate Immunity Protein Tag7 (PGLYRP1) to Cytotoxic Effector Lymphocytes

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  Doi: 10.5281/zenodo.4292193
  Link: https://zenodo.org/record/4292193#.X7-wD9UzbIU
  Description: Supplementary Fig. S1. Changes in cytotoxic activity of different lymphocyte subpopulations induced in PBMC incubated with Tag7 (10–9M) for different periods of time. Lymphocyte subpopulations were isolated by magnetic cell separation and tested for cytotoxicity against K562 cells. Data are presented as the mean ± SD of 3 independent experiments. Differences from the control in all cases are significant at * p < 0.03, ** p < 0.05 (Student t test). Supplementary Fig. S2. Secretion of IL-2 by CD3+CD4+ - lymphocytes incubated with TNFα or IFNγ for 24 and 48 hours. CD3+CD4+ - lymphocytes were isolated by magnetic cell separation from PBMC on 0 day and treated with TNFα or IFNγ. Conditioned medium from untreated lymphocytes was used as a control. The medium was sampled every 24 h to determine IL-2 level by ELISA. Data are presented as the mean ± SD of 3 independent experiments. Differences from the control in all cases are significant at * p < 0.05 (2-way ANOVA). Supplementary Fig. S3. Cytotoxicity Tag7-PBMC in presense specific antibodies to TNFα, IFNγ, IL-2. Each antibody (1:1000) was added to PBMC before 1h to Tag7 (10-9M). After 6 days of incubation PBMC were tested for cytotoxicity against K562 cells. Data are presented as the mean ± SD of 3 independent experiments. Differences from the control in all cases are significant at * p < 0.05 (Student t test).

Sharapova, T.N.; Romanova, E.A.; Ivanova, O.K.; Sashchenko, L.P.; Yashin, D.V. Cytokines TNFα, IFNγ and IL-2 Are Responsible for Signal Transmission from the Innate Immunity Protein Tag7 (PGLYRP1) to Cytotoxic Effector Lymphocytes. Cells 2020, 9, 2602. https://doi.org/10.3390/cells9122602