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Open AccessArticle

Characterization of In Vitro 3D Cell Model Developed from Human Hepatocellular Carcinoma (HepG2) Cell Line

1
Department of Genetic Toxicology and Cancer Biology, National Institute of Biology, 1000 Ljubljana, Slovenia
2
Jozef Stefan International Postgraduate School, 1000 Ljubljana, Slovenia
*
Author to whom correspondence should be addressed.
Cells 2020, 9(12), 2557; https://doi.org/10.3390/cells9122557
Received: 23 October 2020 / Revised: 23 November 2020 / Accepted: 26 November 2020 / Published: 28 November 2020
In genetic toxicology, there is a trend against the increased use of in vivo models as highlighted by the 3R strategy, thus encouraging the development and implementation of alternative models. Two-dimensional (2D) hepatic cell models, which are generally used for studying the adverse effects of chemicals and consumer products, are prone to giving misleading results. On the other hand, newly developed hepatic three-dimensional (3D) cell models provide an attractive alternative, which, due to improved cell interactions and a higher level of liver-specific functions, including metabolic enzymes, reflect in vivo conditions more accurately. We developed an in vitro 3D cell model from the human hepatocellular carcinoma (HepG2) cell line. The spheroids were cultured under static conditions and characterised by monitoring their growth, morphology, and cell viability during the time of cultivation. A time-dependent suppression of cell division was observed. Cell cycle analysis showed time-dependent accumulation of cells in the G0/G1 phase. Moreover, time-dependent downregulation of proliferation markers was shown at the mRNA level. Genes encoding hepatic markers, metabolic phase I/II enzymes, were time-dependently deregulated compared to monolayers. New knowledge on the characteristics of the 3D cell model is of great importance for its further development and application in the safety assessment of chemicals, food products, and complex mixtures. View Full-Text
Keywords: 3D cell model; HepG2; cell proliferation; cell cycle; gene expression 3D cell model; HepG2; cell proliferation; cell cycle; gene expression
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MDPI and ACS Style

Štampar, M.; Breznik, B.; Filipič, M.; Žegura, B. Characterization of In Vitro 3D Cell Model Developed from Human Hepatocellular Carcinoma (HepG2) Cell Line. Cells 2020, 9, 2557. https://doi.org/10.3390/cells9122557

AMA Style

Štampar M, Breznik B, Filipič M, Žegura B. Characterization of In Vitro 3D Cell Model Developed from Human Hepatocellular Carcinoma (HepG2) Cell Line. Cells. 2020; 9(12):2557. https://doi.org/10.3390/cells9122557

Chicago/Turabian Style

Štampar, Martina; Breznik, Barbara; Filipič, Metka; Žegura, Bojana. 2020. "Characterization of In Vitro 3D Cell Model Developed from Human Hepatocellular Carcinoma (HepG2) Cell Line" Cells 9, no. 12: 2557. https://doi.org/10.3390/cells9122557

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