The Primary Cilium of Adipose Progenitors Is Necessary for Their Differentiation into Cancer-Associated Fibroblasts that Promote Migration of Breast Cancer Cells In Vitro
Abstract
1. Introduction
2. Materials and Methods
2.1. Material
2.2. Cell Culture
2.2.1. APs
2.2.2. BCCs
2.3. siRNA
2.4. Immunocytochemistry
2.5. Wound Healing Assay
2.6. Western Blot
2.7. RNA Extraction and Analysis
2.8. Statistics
3. Results
3.1. A Semi Co-Incubation Technique to Study APs Conversion into CAFs
3.2. Conversion of APs into CAFs by BCCs Is Dependent upon the Primary Cilium
3.3. Conversion of APs into CAFs by BCCs Is Dependent upon TGF-β1
3.4. Deciliation of APs Decrease Their Ability to Enhance BCCs Migration, But Not Proliferation
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Peraldi, P.; Ladoux, A.; Giorgetti-Peraldi, S.; Dani, C. The Primary Cilium of Adipose Progenitors Is Necessary for Their Differentiation into Cancer-Associated Fibroblasts that Promote Migration of Breast Cancer Cells In Vitro. Cells 2020, 9, 2251. https://doi.org/10.3390/cells9102251
Peraldi P, Ladoux A, Giorgetti-Peraldi S, Dani C. The Primary Cilium of Adipose Progenitors Is Necessary for Their Differentiation into Cancer-Associated Fibroblasts that Promote Migration of Breast Cancer Cells In Vitro. Cells. 2020; 9(10):2251. https://doi.org/10.3390/cells9102251
Chicago/Turabian StylePeraldi, Pascal, Annie Ladoux, Sophie Giorgetti-Peraldi, and Christian Dani. 2020. "The Primary Cilium of Adipose Progenitors Is Necessary for Their Differentiation into Cancer-Associated Fibroblasts that Promote Migration of Breast Cancer Cells In Vitro" Cells 9, no. 10: 2251. https://doi.org/10.3390/cells9102251
APA StylePeraldi, P., Ladoux, A., Giorgetti-Peraldi, S., & Dani, C. (2020). The Primary Cilium of Adipose Progenitors Is Necessary for Their Differentiation into Cancer-Associated Fibroblasts that Promote Migration of Breast Cancer Cells In Vitro. Cells, 9(10), 2251. https://doi.org/10.3390/cells9102251