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Open AccessArticle

Visfatin Induces Senescence of Human Dental Pulp Cells

1
Department of Dental Pharmacology, BK21 PLUS Project, School of Dentistry, Pusan National University, Yangsan 50612, Korea
2
Periodontal Disease Signaling Network Research Center, School of Dentistry, Pusan National University, Yangsan 50612, Korea
3
Tokuyama University, Shunan, Yamaguchi 745-8566, Japan
4
Department of Oral Physiology, BK21 PLUS Project, School of Dentistry, Pusan National University, Yangsan 50612, Korea
5
Dental and Life Science Institute, School of Dentistry, Pusan National University, Yangsan 50612, Korea
6
Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Yangsan 50612, Korea
7
Department of Oral Pathology, BK21 PLUS Project, School of Dentistry, Pusan National University, Yangsan 50612, Korea
*
Author to whom correspondence should be addressed.
Denotes co-first authors.
Cells 2020, 9(1), 193; https://doi.org/10.3390/cells9010193
Received: 6 November 2019 / Revised: 9 January 2020 / Accepted: 10 January 2020 / Published: 12 January 2020
Dental pulp plays an important role in the health of teeth. The aging of teeth is strongly related to the senescence of dental pulp cells. A novel adipokine, visfatin, is closely associated with cellular senescence. However, little is known about the effect of visfatin on the senescence of human dental pulp cells (hDPCs). Here, it was found that in vivo visfatin levels in human dental pulp tissues increase with age and are upregulated in vitro in hDPCs during premature senescence activated by H2O2, suggesting a correlation between visfatin and senescence. In addition, visfatin knockdown by small interfering RNA led to the reduction in hDPC senescence; however, treatment with exogenous visfatin protein induced the senescence of hDPCs along with increased NADPH consumption, which was reversed by FK866, a chemical inhibitor of visfatin. Furthermore, visfatin-induced senescence was associated with both the induction of telomere damage and the upregulation of senescence-associated secretory phenotype (SASP) factors as well as NF-κB activation, which were all inhibited by FK866. Taken together, these results demonstrate, for the first time, that visfatin plays a pivotal role in hDPC senescence in association with telomere dysfunction and the induction of SASP factors. View Full-Text
Keywords: visfatin; senescence; dental pulp cells; telomere damage; SASP factors; NF-κB; inflammation visfatin; senescence; dental pulp cells; telomere damage; SASP factors; NF-κB; inflammation
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MDPI and ACS Style

Ok, C.Y.; Park, S.; Jang, H.-O.; Takata, T.; Bae, M.-K.; Kim, Y.-D.; Ryu, M.H.; Bae, S.-K. Visfatin Induces Senescence of Human Dental Pulp Cells. Cells 2020, 9, 193.

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