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Article

Inhibition of Transcription Induces Phosphorylation of YB-1 at Ser102 and Its Accumulation in the Nucleus

1
Institute of Protein Research, Russian Academy of Sciences, Pushchino 142290, Russia
2
SABNP, Univ.Evry, INSERM U1204, Université Paris-Saclay, 91025 Evry, France
3
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences (SB RAS), Novosibirsk 630090, Russia
*
Authors to whom correspondence should be addressed.
Contributed equally to this work.
Current address: Boston University School of Medicine, Boston, MA 02118, USA.
Cells 2020, 9(1), 104; https://doi.org/10.3390/cells9010104
Received: 25 November 2019 / Revised: 26 December 2019 / Accepted: 30 December 2019 / Published: 31 December 2019
(This article belongs to the Section Cell Nuclei: Function, Transport and Receptors)
The Y-box binding protein 1 (YB-1) is an RNA/DNA-binding protein regulating gene expression in the cytoplasm and the nucleus. Although mostly cytoplasmic, YB-1 accumulates in the nucleus under stress conditions. Its nuclear localization is associated with aggressiveness and multidrug resistance of cancer cells, which makes the understanding of the regulatory mechanisms of YB-1 subcellular distribution essential. Here, we report that inhibition of RNA polymerase II (RNAPII) activity results in the nuclear accumulation of YB-1 accompanied by its phosphorylation at Ser102. The inhibition of kinase activity reduces YB-1 phosphorylation and its accumulation in the nucleus. The presence of RNA in the nucleus is shown to be required for the nuclear retention of YB-1. Thus, the subcellular localization of YB-1 depends on its post-translational modifications (PTMs) and intracellular RNA distribution. View Full-Text
Keywords: YB-1; nuclear translocation; inhibition of transcription; phosphorylation YB-1; nuclear translocation; inhibition of transcription; phosphorylation
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MDPI and ACS Style

Kretov, D.A.; Mordovkina, D.A.; Eliseeva, I.A.; Lyabin, D.N.; Polyakov, D.N.; Joshi, V.; Desforges, B.; Hamon, L.; Lavrik, O.I.; Pastré, D.; Curmi, P.A.; Ovchinnikov, L.P. Inhibition of Transcription Induces Phosphorylation of YB-1 at Ser102 and Its Accumulation in the Nucleus. Cells 2020, 9, 104. https://doi.org/10.3390/cells9010104

AMA Style

Kretov DA, Mordovkina DA, Eliseeva IA, Lyabin DN, Polyakov DN, Joshi V, Desforges B, Hamon L, Lavrik OI, Pastré D, Curmi PA, Ovchinnikov LP. Inhibition of Transcription Induces Phosphorylation of YB-1 at Ser102 and Its Accumulation in the Nucleus. Cells. 2020; 9(1):104. https://doi.org/10.3390/cells9010104

Chicago/Turabian Style

Kretov, Dmitry A., Daria A. Mordovkina, Irina A. Eliseeva, Dmitry N. Lyabin, Dmitry N. Polyakov, Vandana Joshi, Bénédicte Desforges, Loic Hamon, Olga I. Lavrik, David Pastré, Patrick A. Curmi, and Lev P. Ovchinnikov 2020. "Inhibition of Transcription Induces Phosphorylation of YB-1 at Ser102 and Its Accumulation in the Nucleus" Cells 9, no. 1: 104. https://doi.org/10.3390/cells9010104

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