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Article

Reappraisal of Human HOG and MO3.13 Cell Lines as a Model to Study Oligodendrocyte Functioning

1
Department of Molecular Animal Physiology, Donders Institute for Brain, Cognition and Behaviour, Centre for Neuroscience, Faculty of Science, Radboud University, 6525AJ Nijmegen, The Netherlands
2
NeuroDrug Research, 6525 HP Nijmegen, The Netherlands
*
Author to whom correspondence should be addressed.
Cells 2019, 8(9), 1096; https://doi.org/10.3390/cells8091096
Received: 4 June 2019 / Revised: 9 September 2019 / Accepted: 10 September 2019 / Published: 17 September 2019
(This article belongs to the Special Issue The Molecular and Cellular Basis for Parkinson's Disease)
Myelination of neuronal axons is essential for proper brain functioning and requires mature myelinating oligodendrocytes (myOLs). The human OL cell lines HOG and MO3.13 have been widely used as in vitro models to study OL (dys) functioning. Here we applied a number of protocols aimed at differentiating HOG and MO3.13 cells into myOLs. However, none of the differentiation protocols led to increased expression of terminal OL differentiation or myelin-sheath formation markers. Surprisingly, the applied protocols did cause changes in the expression of markers for early OLs, neurons, astrocytes and Schwann cells. Furthermore, we noticed that mRNA expression levels in HOG and MO3.13 cells may be affected by the density of the cultured cells. Finally, HOG and MO3.13 co-cultured with human neuronal SH-SY5Y cells did not show myelin formation under several pro-OL-differentiation and pro-myelinating conditions. Together, our results illustrate the difficulty of inducing maturation of HOG and MO3.13 cells into myOLs, implying that these oligodendrocytic cell lines may not represent an appropriate model to study the (dys)functioning of human (my)OLs and OL-linked disease mechanisms. View Full-Text
Keywords: cell line; differentiation; HOG; immature oligodendrocyte; Krabbe’s disease; oligodendrocyte; mature oligodendrocyte; MO3.13; myelin; multiple sclerosis; schizophrenia; SH-SY5Y cell line; differentiation; HOG; immature oligodendrocyte; Krabbe’s disease; oligodendrocyte; mature oligodendrocyte; MO3.13; myelin; multiple sclerosis; schizophrenia; SH-SY5Y
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MDPI and ACS Style

De Kleijn, K.M.A.; Zuure, W.A.; Peijnenborg, J.; Heuvelmans, J.M.; Martens, G.J.M. Reappraisal of Human HOG and MO3.13 Cell Lines as a Model to Study Oligodendrocyte Functioning. Cells 2019, 8, 1096. https://doi.org/10.3390/cells8091096

AMA Style

De Kleijn KMA, Zuure WA, Peijnenborg J, Heuvelmans JM, Martens GJM. Reappraisal of Human HOG and MO3.13 Cell Lines as a Model to Study Oligodendrocyte Functioning. Cells. 2019; 8(9):1096. https://doi.org/10.3390/cells8091096

Chicago/Turabian Style

De Kleijn, Kim M.A.; Zuure, Wieteke A.; Peijnenborg, Jolien; Heuvelmans, Josje M.; Martens, Gerard J.M. 2019. "Reappraisal of Human HOG and MO3.13 Cell Lines as a Model to Study Oligodendrocyte Functioning" Cells 8, no. 9: 1096. https://doi.org/10.3390/cells8091096

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