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Article

DROSHA-Dependent AIM2 Inflammasome Activation Contributes to Lung Inflammation during Idiopathic Pulmonary Fibrosis

1
Joan and Sanford I. Weill Department of Medicine, Weill Cornell Medical College, New York-Presbyterian Hospital, New York, NY 10011, USA
2
Division of Pulmonary and Critical Care Medicine, Weill Cornell Medical College, New York, NY 10011, USA
3
Department of Surgery and Critical Care Medicine, Ewha Womans University College of Medicine, Seoul 05505, Korea
4
Department of Integrated Biomedical Science, Soonchunhyang Institute of Medi-bio Science (SIMS), Soonchunhyang University, Cheonan-si 31151, Korea
*
Author to whom correspondence should be addressed.
Cells 2019, 8(8), 938; https://doi.org/10.3390/cells8080938
Received: 22 July 2019 / Revised: 18 August 2019 / Accepted: 19 August 2019 / Published: 20 August 2019
(This article belongs to the Special Issue Mechanisms of Inflammasome Activation)
Idiopathic pulmonary fibrosis (IPF) has been linked to chronic lung inflammation. Drosha ribonuclease III (DROSHA), a class 2 ribonuclease III enzyme, plays a key role in microRNA (miRNA) biogenesis. However, the mechanisms by which DROSHA affects the lung inflammation during idiopathic pulmonary fibrosis (IPF) remain unclear. Here, we demonstrate that DROSHA regulates the absent in melanoma 2 (AIM2) inflammasome activation during idiopathic pulmonary fibrosis (IPF). Both DROSHA and AIM2 protein expression were elevated in alveolar macrophages of patients with IPF. We also found that DROSHA and AIM2 protein expression were increased in alveolar macrophages of lung tissues in a mouse model of bleomycin-induced pulmonary fibrosis. DROSHA deficiency suppressed AIM2 inflammasome-dependent caspase-1 activation and interleukin (IL)-1β and IL-18 secretion in primary mouse alveolar macrophages and bone marrow-derived macrophages (BMDMs). Transduction of microRNA (miRNA) increased the formation of the adaptor apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) specks, which is required for AIM2 inflammasome activation in BMDMs. Our results suggest that DROSHA promotes AIM2 inflammasome activation-dependent lung inflammation during IPF. View Full-Text
Keywords: DROSHA; miRNA; AIM2 inflammasome; idiopathic pulmonary fibrosis DROSHA; miRNA; AIM2 inflammasome; idiopathic pulmonary fibrosis
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MDPI and ACS Style

Cho, S.J.; Hong, K.S.; Jeong, J.H.; Lee, M.; Choi, A.M.K.; Stout-Delgado, H.W.; Moon, J.-S. DROSHA-Dependent AIM2 Inflammasome Activation Contributes to Lung Inflammation during Idiopathic Pulmonary Fibrosis. Cells 2019, 8, 938. https://doi.org/10.3390/cells8080938

AMA Style

Cho SJ, Hong KS, Jeong JH, Lee M, Choi AMK, Stout-Delgado HW, Moon J-S. DROSHA-Dependent AIM2 Inflammasome Activation Contributes to Lung Inflammation during Idiopathic Pulmonary Fibrosis. Cells. 2019; 8(8):938. https://doi.org/10.3390/cells8080938

Chicago/Turabian Style

Cho, Soo J., Kyoung S. Hong, Ji H. Jeong, Mihye Lee, Augustine M.K. Choi, Heather W. Stout-Delgado, and Jong-Seok Moon. 2019. "DROSHA-Dependent AIM2 Inflammasome Activation Contributes to Lung Inflammation during Idiopathic Pulmonary Fibrosis" Cells 8, no. 8: 938. https://doi.org/10.3390/cells8080938

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