DROSHA-Dependent AIM2 Inflammasome Activation Contributes to Lung Inflammation during Idiopathic Pulmonary Fibrosis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Human Subjects
2.2. Animal Studies
2.3. Reagents and Antibodies
2.4. Cell Culture
2.5. Transduction of Drosha gRNA/Cas9 pPlasmid or Drosha Small Interfering RNA (siRNA)
2.6. Synthetic miRNAs
2.7. Immunoblot Analysis
2.8. Immunohistochemistry and Immunofluorescence Analysis
2.9. Cytokine Analysis
2.10. ASC Speck Formation Assay
2.11. Statistical Analysis
3. Results
3.1. The DROSHA and AIM2 Protein Levels were Elevated in Lung Tissues of Patients with IPF
3.2. The DROSHA and AIM2 Expression Levels were Elevated in Alveolar Macrophages of Patients with IPF
3.3. The DROSHA Protein Levels were Elevated in Alveolar Macrophages during Bleomycin-Induced Pulmonary Fibrosis
3.4. Deficiency of DROSHA Suppresses the AIM2 Inflammasome Activation in Alveolar Macrophages
3.5. Deficiency of DROSHA Suppresses the AIM2 Inflammasome Activation in Macrophages
3.6. Transduction of miRNA Promotes the ASC Speck Formation for AIM2 Inflammasome Activation
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Control | IPF | |
---|---|---|
Subjects | 12 | 12 |
Age years | 46.5+/−11.4 | 65+/−9.2 |
Male/female | 11/1 | 11/1 |
Smoking status yes/no | 4/8 | 6/6 |
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Cho, S.J.; Hong, K.S.; Jeong, J.H.; Lee, M.; Choi, A.M.K.; Stout-Delgado, H.W.; Moon, J.-S. DROSHA-Dependent AIM2 Inflammasome Activation Contributes to Lung Inflammation during Idiopathic Pulmonary Fibrosis. Cells 2019, 8, 938. https://doi.org/10.3390/cells8080938
Cho SJ, Hong KS, Jeong JH, Lee M, Choi AMK, Stout-Delgado HW, Moon J-S. DROSHA-Dependent AIM2 Inflammasome Activation Contributes to Lung Inflammation during Idiopathic Pulmonary Fibrosis. Cells. 2019; 8(8):938. https://doi.org/10.3390/cells8080938
Chicago/Turabian StyleCho, Soo Jung, Kyoung Sook Hong, Ji Hun Jeong, Mihye Lee, Augustine M. K. Choi, Heather W. Stout-Delgado, and Jong-Seok Moon. 2019. "DROSHA-Dependent AIM2 Inflammasome Activation Contributes to Lung Inflammation during Idiopathic Pulmonary Fibrosis" Cells 8, no. 8: 938. https://doi.org/10.3390/cells8080938