Connecting the Dots in the Neuroglobin-Protein Interaction Network of an Unstressed and Ferroptotic Cell Death Neuroblastoma Model
Abstract
:1. Introduction
2. Materials and Methods
2.1. Stable Cell Line Generation
2.2. Flow-Cytometric Phenotyping and Analysis of Cell Viability
2.3. Lipid Peroxidation
2.4. IncuCyte Kinetic Monitoring of Cell Death
2.5. RNA Extraction and Reverse Transcription Quantitative PCR
2.6. Protein-Protein Interactions
2.6.1. Cell Culturing
2.6.2. Co-Immunoprecipitation
2.6.3. Protein Digestion
2.6.4. LC-MS/MS Analysis
2.6.5. Mass Spectrometric Data Analysis
2.7. Statistical Analysis
3. Results
3.1. Characterization and Validation of a hNgb-EGFP SH-SY5Y Cell Line
3.2. hNgb-EGFP SH-SY5Y Cells Have a Higher Threshold for Ferroptosis Initiation
3.3. Protein Interactors of hNgb in a Non-Stress Condition are Enriched in the ‘Cell Death and Survival, Cellular Development, Cellular Growth, and Proliferation’ Network
3.4. SILAC Labeling Shows Ferroptosis-Specific hNgb Interactions
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Van Acker, Z.P.; Van Raemdonck, G.A.; Logie, E.; Van Acker, S.I.; Baggerman, G.; Vanden Berghe, W.; Ponsaerts, P.; Dewilde, S. Connecting the Dots in the Neuroglobin-Protein Interaction Network of an Unstressed and Ferroptotic Cell Death Neuroblastoma Model. Cells 2019, 8, 873. https://doi.org/10.3390/cells8080873
Van Acker ZP, Van Raemdonck GA, Logie E, Van Acker SI, Baggerman G, Vanden Berghe W, Ponsaerts P, Dewilde S. Connecting the Dots in the Neuroglobin-Protein Interaction Network of an Unstressed and Ferroptotic Cell Death Neuroblastoma Model. Cells. 2019; 8(8):873. https://doi.org/10.3390/cells8080873
Chicago/Turabian StyleVan Acker, Zoë P., Geert A. Van Raemdonck, Emilie Logie, Sara I. Van Acker, Geert Baggerman, Wim Vanden Berghe, Peter Ponsaerts, and Sylvia Dewilde. 2019. "Connecting the Dots in the Neuroglobin-Protein Interaction Network of an Unstressed and Ferroptotic Cell Death Neuroblastoma Model" Cells 8, no. 8: 873. https://doi.org/10.3390/cells8080873