The Ubiquitin Moiety of Ubi1 Is Required for Productive Expression of Ribosomal Protein eL40 in Saccharomyces cerevisiae
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains and Microbiological Methods
2.2. Plasmids
2.3. Polysome Analysis and Sucrose Gradient Fractionation
2.4. Western Blot Analysis and Antibodies
2.5. Analysis of Aggregated Proteins
2.6. Reproducibility
3. Results
3.1. Generation of ubi1 Mutants for Phenotypic Analysis
3.2. The ubi1∆ub Ubiquitin Deletion Mutant Displays a Slow-Growth Phenotype
3.3. The SMT3-S-eL40A ubi2∆ Mutant Displays a Slow-Growth Phenotype
3.4. Contribution of the Ubiquitin and SUMO Moieties to the Expression of eL40A
3.5. The Genomic ubi1∆ub-HA and SMT3-S-eL40A-HA Alleles Affect the Functional Integrity of 60S r-Subunits
3.6. In Trans Expression of Free Ubiquitin Ffails to Rescue the Deficiencies of the ubi1∆ub-HA ubi2∆ and SMT3-S-eL40A-HA ubi2∆ Mutants
3.7. Ubiquitin and SUMO Modestly Prevent eL40 or eS31 Protein Aggregation
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Strain a | Relevant Genotype b | Source |
---|---|---|
W303-1A | MATaade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 | [37] |
W303-1B | As W303-1A but MATα | [37] |
TAY001 | MATaubi1::kanMX4 ubi2::kanMX4 ade3::kanMX4 [pHT4467∆-UBI1] | [36] |
SMY106 | MATaubi1::klURA3 | This study |
SMY113 | MATa UBI1-HA | This study |
SMY256 | MATaUBI1-HA ubi2::kanMX4 | This study |
SMY107 | MATaubi1∆ub-HA | This study |
SMY257 | MATα ubi1∆ub-HA ubi2::kanMX4 | This study |
SMY215 | MATaubi1::kanMX4 ubi2::kanMX4 ade3::kanMX4 [YCplac111-ubi1∆ub-HA] | This study |
JDY923 | MATα ubi2::kanMX4 | [33] |
SMY218 | MATaSMT3-S-eL40A-HA | This study |
SMY258 | MATα SMT3-S-eL40A-HA ubi2::kanMX4 | This study |
SMY216 | MATaubi1::kanMX4 ubi2::kanMX4 ade3::kanMX4 [YCplac111-SMT3-S-eL40A-HA] | This study |
JDY532 | MATassb1::HIS3MX6 ssb2::natNT2 | This study |
SMY324 | MATassb1::HIS3MX6 ssb2::natNT2 UBI3-HA::kanMX4 | This study |
TLY56.D3 | MATaUBI3-HA::kanMX4 | [35] |
TLY61.A2 | MATaubi3∆ub-HA::kanMX4 | [35] |
TLY14.3C | MATα ubi3::HIS3MX6 | [35] |
SMY315 | MATα rps12::kanMX4 | This study |
ORY211 | MATα rpl39::natNT2 | This study |
MS157-1A | MATα dob1-1 | [42] |
Name (Collection Name) | Relevant Information | Source |
---|---|---|
pHT4467∆-UBI1 | CEN6 (instable), URA3, ADE3. Wild-type Ubi1; promoter and terminator of UBI1 | [36] |
YCplac111 | CEN, LEU2 | [43] |
YCplac111-UBI1-HA (pDK4131) | CEN, LEU2. C-terminally 1xHA-tagged eL40A; promoter and terminator of UBI1 | [36] |
YCplac111-ubi1∆ub-HA (pDK4192) | CEN, LEU2. Allele ubi1∆ub-HA (M-II); promoter and terminator of UBI1 | This study |
YCplac111-SMT3-S-eL40-HA (pDK4193) | CEN, LEU2. Allele SMT3-S-eL40A-HA (GG-S-II); promoter and terminator of UBI1 | This study |
pADH111-Ub (pDK2253) | CEN, LEU2. Wild-type ubiquitin; promoter and terminator of ADH1 | This study |
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Martín-Villanueva, S.; Fernández-Pevida, A.; Kressler, D.; de la Cruz, J. The Ubiquitin Moiety of Ubi1 Is Required for Productive Expression of Ribosomal Protein eL40 in Saccharomyces cerevisiae. Cells 2019, 8, 850. https://doi.org/10.3390/cells8080850
Martín-Villanueva S, Fernández-Pevida A, Kressler D, de la Cruz J. The Ubiquitin Moiety of Ubi1 Is Required for Productive Expression of Ribosomal Protein eL40 in Saccharomyces cerevisiae. Cells. 2019; 8(8):850. https://doi.org/10.3390/cells8080850
Chicago/Turabian StyleMartín-Villanueva, Sara, Antonio Fernández-Pevida, Dieter Kressler, and Jesús de la Cruz. 2019. "The Ubiquitin Moiety of Ubi1 Is Required for Productive Expression of Ribosomal Protein eL40 in Saccharomyces cerevisiae" Cells 8, no. 8: 850. https://doi.org/10.3390/cells8080850