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Poc1B and Sas-6 Function Together during the Atypical Centriole Formation in Drosophila melanogaster

Department of Biological Sciences, University of Toledo, Toledo, OH 43607, USA
*
Author to whom correspondence should be addressed.
Cells 2019, 8(8), 841; https://doi.org/10.3390/cells8080841
Received: 10 June 2019 / Revised: 25 July 2019 / Accepted: 29 July 2019 / Published: 5 August 2019
(This article belongs to the Section Organelle Function)
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Abstract

Insects and mammals have atypical centrioles in their sperm. However, it is unclear how these atypical centrioles form. Drosophila melanogaster sperm has one typical centriole called the giant centriole (GC) and one atypical centriole called the proximal centriole-like structure (PCL). During early sperm development, centriole duplication factors such as Ana2 and Sas-6 are recruited to the GC base to initiate PCL formation. The centriolar protein, Poc1B, is also recruited at this initiation stage, but its precise role during PCL formation is unclear. Here, we show that Poc1B recruitment was dependent on Sas-6, that Poc1B had effects on cellular and PCL Sas-6, and that Poc1B and Sas-6 were colocalized in the PCL/centriole core. These findings suggest that Sas-6 and Poc1B interact during PCL formation. Co-overexpression of Ana2 and Sas-6 induced the formation of ectopic particles that contained endogenous Poc1 proteins and were composed of PCL-like structures. These structures were disrupted in Poc1 mutant flies, suggesting that Poc1 proteins stabilize the PCL-like structures. Lastly, Poc1B and Sas-6 co-overexpression also induced the formation of PCL-like structures, suggesting that they can function together during the formation of the PCL. Overall, our findings suggest that Poc1B and Sas-6 function together during PCL formation. View Full-Text
Keywords: centriole; centrosome; sperm; proximal centriole-like structure (PCL); atypical centriole; cilium; flagella; microtubule-organizing center (MTOC); Poc1B; Sas-6 centriole; centrosome; sperm; proximal centriole-like structure (PCL); atypical centriole; cilium; flagella; microtubule-organizing center (MTOC); Poc1B; Sas-6
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Jo, K.H.; Jaiswal, A.; Khanal, S.; Fishman, E.L.; Curry, A.N.; Avidor-Reiss, T. Poc1B and Sas-6 Function Together during the Atypical Centriole Formation in Drosophila melanogaster. Cells 2019, 8, 841.

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