CDDO-Me Selectively Attenuates CA1 Neuronal Death Induced by Status Epilepticus via Facilitating Mitochondrial Fission Independent of LONP1
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Animals and Chemicals
2.2. Surgery, CDDO-Me Infusion and LONP1 Knockdown
2.3. SE Induction and Electroencephalogram (EEG) Analysis
2.4. Tissue Processing
2.5. Immunohistochemistry and Measurements of Mitochondrial Length and Neuronal Damage
2.6. Western Blot
2.7. Quantification of Data and Statistical Analysis
3. Results
3.1. CDDO-Me Distinctly Affects SE-Induced Neuronal Death in the Hippocampus
3.2. CDDO-Me Induces Mitochondrial Fission in CA1 Neuron and PV Cells without Altering LONP1 Expression
3.3. CDDO-Me Enhances DRP1-S616 Phosphorylation in Control- and Post-SE Animals
3.4. CDDO-Me Increases ERK1/2 and JNK Activities in the Hippocampus
3.5. Effects of CDDO-Me on SE-Induced Neuronal Death and Mitochondrial Dynamics are Independent of LONP1 Activity
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Antigen | Host | Manufacturer (Catalog Number) | Dilution Used |
---|---|---|---|
DRP1 | Rabbit | Thermo (PA1-16987) | 1:1000 (WB) |
ERK1/2 | Rabbit | Biorbyt (Orb160960) | 1:2000 (WB) |
JNK | Rabbit | Protein tech (10023-1-AP) | 1:1000 (WB) |
LONP1 | Rabbit | Proteintech (15440-1-AP) | 1:100 (IF) 1:1000 (WB) |
MFN1 | Rabbit | Proteintech (13798-1-AP) | 1:1000 (WB) |
MFN2 | Rabbit | Proteintech (12186-1-AP) | 1:1000 (WB) |
Mitochondrial marker (Mitochondrial complex IV subunit 1, MTCO1) | Mouse | Abcam (#ab14705) | 1:500 (IF) |
OPA1 | Rabbit | Abcam (ab42364) | 1:1000 (WB) |
pDRP1 S616 | Rabbit | Cell Signaling (4494) | 1:500 (WB) |
pDRP1 S637 | Rabbit | Cell Signaling (4867) | 1:500 (WB) |
pERK1/2 | Rabbit | Bioss (bs-3330R) | 1:1000 (WB) |
PKA catalytic subunit | Rabbit | BioVision (3115-100) | 1:1000 (WB) |
PKA regulatory subunit | Rabbit | Santa Cruz (sc-909) | 1:1000 (WB) |
PP1 | Rabbit | Abcam (ab52619) | 1:5000 (WB) |
PP2A | Rabbit | Cell Signaling (#2038) | 1:5000 (WB) |
PP2B | Rabbit | Millipore (07-068-I) | 1:1000 (WB) |
pPKA catalytic subunit | Rabbit | Assay Biotec (A0548) | 1:1000 (WB) |
pPKA regulatory subunit | Rabbit | GeneTex (GTX61061) | 1:2500 (WB) |
pPP1 | Rabbit | Abcam (ab62334) | 1:5000 (WB) |
pPP2A | Rabbit | Sigma (SAB4503975) | 1:1000 (WB) |
pPP2B | Rabbit | Badrilla (A010-80) | 1:1000 (WB) |
PV | Goat | Swant (#PVG213) | 1:10,000 (IF) |
β-actin | Mouse | Sigma (A5316) | 1:5000 (WB) |
4-HNE | Rabbit | Alpha Diagnostic (# HNE11-S) | 1:1000 (IF) |
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Kim, J.-E.; Park, H.; Choi, S.-H.; Kong, M.-J.; Kang, T.-C. CDDO-Me Selectively Attenuates CA1 Neuronal Death Induced by Status Epilepticus via Facilitating Mitochondrial Fission Independent of LONP1. Cells 2019, 8, 833. https://doi.org/10.3390/cells8080833
Kim J-E, Park H, Choi S-H, Kong M-J, Kang T-C. CDDO-Me Selectively Attenuates CA1 Neuronal Death Induced by Status Epilepticus via Facilitating Mitochondrial Fission Independent of LONP1. Cells. 2019; 8(8):833. https://doi.org/10.3390/cells8080833
Chicago/Turabian StyleKim, Ji-Eun, Hana Park, Seo-Hyeon Choi, Min-Jeong Kong, and Tae-Cheon Kang. 2019. "CDDO-Me Selectively Attenuates CA1 Neuronal Death Induced by Status Epilepticus via Facilitating Mitochondrial Fission Independent of LONP1" Cells 8, no. 8: 833. https://doi.org/10.3390/cells8080833