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Open AccessArticle

Overexpression of MiR482c in Tomato Induces Enhanced Susceptibility to Late Blight

School of Bioengineering, Dalian University of Technology, Dalian 116024, China
School of Computer Science and Technology, Dalian University of Technology, Dalian 116024, China
Author to whom correspondence should be addressed.
Cells 2019, 8(8), 822;
Received: 9 July 2019 / Revised: 30 July 2019 / Accepted: 1 August 2019 / Published: 3 August 2019
(This article belongs to the Section Cell Nuclei: Function, Transport and Receptors)
PDF [2203 KB, uploaded 3 August 2019]


Tomato is the highest-value fruit/vegetable crop worldwide. However, the quality and yield of tomatoes are severely affected by late blight. MicroRNA482s (miR482s) are involved in the plant’s immune system. In this study, miR482c was transiently and stably overexpressed in tomatoes in transgenic plants to explore its mechanism in tomato resistance against late blight. Transgenic tomato plants with transiently overexpressed miR482c displayed a larger lesion area than the control plants upon infection. Furthermore, compared with wild-type (WT) tomato plants, the transgenic tomato plants stably overexpressing miR482c displayed a decreased expression of target genes accompanied by lower peroxidase (POD), superoxide dismutase (SOD), and phenylalanine ammonia-lyase (PAL) activity activities and higher malondialdehyde (MDA) content, thereby leading to a decline in reactive oxygen species (ROS) scavenging ability and aggravating the damage of lipid peroxidation product accumulation on the cell membrane, eventually enhancing plant susceptibility. This finding indicates that miR482c may act as a negative regulator in tomato resistance by regulating nucleotide binding sites and leucine-rich repeat (NBS-LRR) expression levels and ROS levels. View Full-Text
Keywords: tomato; late blight; miRNA; overexpression; resistance tomato; late blight; miRNA; overexpression; resistance

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Hong, Y.-H.; Meng, J.; He, X.-L.; Zhang, Y.-Y.; Luan, Y.-S. Overexpression of MiR482c in Tomato Induces Enhanced Susceptibility to Late Blight. Cells 2019, 8, 822.

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