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Response of the Green Alga Chlamydomonas reinhardtii to the DNA Damaging Agent Zeocin

Laboratory of Cell Cycles of Algae, Centre Algatech, Institute of Microbiology, Czech Academy of Sciences, 37981 Třeboň, Czech Republic
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These authors contributed equally to this work.
Cells 2019, 8(7), 735; https://doi.org/10.3390/cells8070735
Received: 18 June 2019 / Revised: 14 July 2019 / Accepted: 15 July 2019 / Published: 17 July 2019
(This article belongs to the Special Issue Chlamydomonas Cell Biology)
DNA damage is a ubiquitous threat endangering DNA integrity in all living organisms. Responses to DNA damage include, among others, induction of DNA repair and blocking of cell cycle progression in order to prevent transmission of damaged DNA to daughter cells. Here, we tested the effect of the antibiotic zeocin, inducing double stranded DNA breaks, on the cell cycle of synchronized cultures of the green alga Chlamydomonas reinhardtii. After zeocin application, DNA replication partially occurred but nuclear and cellular divisions were completely blocked. Application of zeocin combined with caffeine, known to alleviate DNA checkpoints, decreased cell viability significantly. This was probably caused by a partial overcoming of the cell cycle progression block in such cells, leading to aberrant cell divisions. The cell cycle block was accompanied by high steady state levels of mitotic cyclin-dependent kinase activity. The data indicate that DNA damage response in C. reinhardtii is connected to the cell cycle block, accompanied by increased and stabilized mitotic cyclin-dependent kinase activity. View Full-Text
Keywords: DNA damage; double-stranded break; zeocin; caffeine; cell cycle; cyclin-dependent kinase; Chlamydomonas reinhardtii DNA damage; double-stranded break; zeocin; caffeine; cell cycle; cyclin-dependent kinase; Chlamydomonas reinhardtii
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Čížková, M.; Slavková, M.; Vítová, M.; Zachleder, V.; Bišová, K. Response of the Green Alga Chlamydomonas reinhardtii to the DNA Damaging Agent Zeocin. Cells 2019, 8, 735.

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