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Open AccessArticle

Cyclic Nucleotide-Specific Optogenetics Highlights Compartmentalization of the Sperm Flagellum into cAMP Microdomains

1
Institute of Innate Immunity, Biophysical Imaging, University Hospital Bonn, University of Bonn, 53127 Bonn, Germany
2
Centrum für Reproduktionsmedizin und Andrologie (CeRA), Universitätsklinikum Münster, Universität Münster, 48129 Münster, Germany
3
Lehrstuhl für Biochemie, Universität Bayreuth, 95447 Bayreuth, Germany
4
Center of Advanced European Studies and Research (caesar), Molecular Sensory Systems, 53175 Bonn, Germany
5
Research Center for Bio-Macromolecules, Universität Bayreuth, 95447 Bayreuth, Germany
6
Bayreuth Center for Biochemistry & Molecular Biology, Universität Bayreuth, 95447 Bayreuth, Germany
7
Center of Advanced European Studies and Research (caesar), Molecular Physiology, 53175 Bonn, Germany
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Cells 2019, 8(7), 648; https://doi.org/10.3390/cells8070648
Received: 21 May 2019 / Revised: 13 June 2019 / Accepted: 25 June 2019 / Published: 27 June 2019
(This article belongs to the Special Issue Cilia and Flagella: Structure, Function and Beyond)
Inside the female genital tract, mammalian sperm undergo a maturation process called capacitation, which primes the sperm to navigate across the oviduct and fertilize the egg. Sperm capacitation and motility are controlled by 3′,5′-cyclic adenosine monophosphate (cAMP). Here, we show that optogenetics, the control of cellular signaling by genetically encoded light-activated proteins, allows to manipulate cAMP dynamics in sperm flagella and, thereby, sperm capacitation and motility by light. To this end, we used sperm that express the light-activated phosphodiesterase LAPD or the photo-activated adenylate cyclase bPAC. The control of cAMP by LAPD or bPAC combined with pharmacological interventions provides spatiotemporal precision and allows to probe the physiological function of cAMP compartmentalization in mammalian sperm. View Full-Text
Keywords: cilia; flagella; optogenetics; cAMP; beat pattern; motility; navigation; sperm cilia; flagella; optogenetics; cAMP; beat pattern; motility; navigation; sperm
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Raju, D.N.; Hansen, J.N.; Rassmann, S.; Stüven, B.; Jikeli, J.F.; Strünker, T.; Körschen, H.G.; Möglich, A.; Wachten, D. Cyclic Nucleotide-Specific Optogenetics Highlights Compartmentalization of the Sperm Flagellum into cAMP Microdomains. Cells 2019, 8, 648.

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