Next Article in Journal
Wound Healing Fluid Reflects the Inflammatory Nature and Aggressiveness of Breast Tumors
Previous Article in Journal
Switching on Endogenous Metal Binding Proteins in Parkinson’s Disease
Previous Article in Special Issue
Cilia Distal Domain: Diversity in Evolutionarily Conserved Structures
Article Menu
Issue 2 (February) cover image

Export Article

Open AccessArticle
Cells 2019, 8(2), 180; https://doi.org/10.3390/cells8020180

The Centriolar Adjunct–Appearance and Disassembly in Spermiogenesis and the Potential Impact on Fertility

1
Faculté de Médecine, Université de Tours, 10, Boulevard Tonnellé, 37032 Tours, France
2
National University of Science and Technology «MISiS», 119049 Moscow, Russia
3
Belozersky Institute of Physico-Chemical Biology, Moscow State University, Leninskye gory 1-40, 119992 Moscow, Russia
4
Research Centre for Medical Genetics, Moskvorechye 1, 115478 Moscow, Russia
5
CHRU, UF Biologie Cellulaire & Microscopie Electronique, 2, Boulevard Tonnellé, 37032 Tours, France
6
INSERM U1259, 10, Boulevard Tonnellé, 37032 Tours, France
7
UFR de Sciences et Techniques, Physiologie de la Reproduction et des Comportements (PRC), INRA, Centre de Tours, 37380 Nouzilly, France
8
Service de Médecine et Biologie de la Reproduction, CHRU Bretonneau, 2, boulevard Tonnellé, 37044 Tours, France
9
Faculty of Bioengineering and Bioinformatics, Moscow State University, Leninskye gory 73, 119992 Moscow, Russia
*
Author to whom correspondence should be addressed.
Received: 16 January 2019 / Revised: 15 February 2019 / Accepted: 16 February 2019 / Published: 19 February 2019
(This article belongs to the Special Issue Cilia and Flagella: Structure, Function and Beyond)
  |  
PDF [6117 KB, uploaded 24 February 2019]
  |     |  

Abstract

During spermiogenesis, the proximal centriole forms a special microtubular structure: the centriolar adjunct. This structure appears at the spermatid stage, which is characterized by a condensed chromatin nucleus. We showed that the centriolar adjunct disappears completely in mature porcine spermatozoa. In humans, the centriolar adjunct remnants are present in a fraction of mature spermatids. For the first time, the structure of the centriolar adjunct in the cell, and its consequent impact on fertility, were examined. Ultrastructural analysis using transmission electron microscopy was performed on near 2000 spermatozoa per person, in two patients with idiopathic male sterility (IMS) and five healthy fertile donors. We measured the average length of the “proximal centriole + centriolar adjunct” complex in sections, where it had parallel orientation in the section plane, and found that it was significantly longer in the spermatozoa of IMS patients than in the spermatozoa of healthy donors. This difference was independent of chromatin condensation deficiency, which was also observed in the spermatozoa of IMS patients. We suggest that zygote arrest may be related to an incompletely disassembled centriolar adjunct in a mature spermatozoon. Therefore, centriolar adjunct length can be potentially used as a complementary criterion for the immaturity of spermatozoa in the diagnostics of IMS patients. View Full-Text
Keywords: centriolar adjunct; centriole; centrosome; idiopathic male sterility (IMS); human spermatozoa ultrastructure; in vitro fertilization (IVF) centriolar adjunct; centriole; centrosome; idiopathic male sterility (IMS); human spermatozoa ultrastructure; in vitro fertilization (IVF)
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Garanina, A.S.; Alieva, I.B.; Bragina, E.E.; Blanchard, E.; Arbeille, B.; Guerif, F.; Uzbekova, S.; Uzbekov, R.E. The Centriolar Adjunct–Appearance and Disassembly in Spermiogenesis and the Potential Impact on Fertility. Cells 2019, 8, 180.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Cells EISSN 2073-4409 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top