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Cytokinesis D is Mediated by Cortical Flow of Dividing Cells Instead of Chemotaxis

1
Graduate School of Sciences and Technology for Innovation, Yamaguchi University, Yamaguchi 753-8511, Japan
2
Department of Biochemistry and Molecular Biology, Faculty of Science, University of Rajshahi, Rajshahi 6205, Bangladesh
3
Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Tokyo 153-8902, Japan
*
Author to whom correspondence should be addressed.
Cells 2019, 8(5), 473; https://doi.org/10.3390/cells8050473
Received: 17 April 2019 / Revised: 14 May 2019 / Accepted: 15 May 2019 / Published: 17 May 2019
(This article belongs to the Special Issue Molecular Factors and Mechanisms Involved in Cytokinesis)
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Abstract

Cytokinesis D is known as the midwife mechanism in which neighboring cells facilitate cell division by crossing the cleavage furrow of dividing cells. Cytokinesis D is thought to be mediated by chemotaxis, where midwife cells migrate toward dividing cells by sensing an unknown chemoattractant secreted from the cleavage furrow. In this study, to validate this chemotaxis model, we aspirated the fluid from the vicinity of the cleavage furrow of a dividing Dictyostelium cell and discharged it onto a neighboring cell using a microcapillary. However, the neighboring cells did not show any chemotaxis toward the fluid. In addition, the cells did not manifest an increase in the levels of intracellular Ca2+, cAMP, or cGMP, which are expected to rise in chemotaxing cells. From several lines of our experiments, including these findings, we concluded that chemotaxis does not contribute to cytokinesis D. As an alternative, we propose a cortical-flow model, where a migrating cell attaches to a dividing cell by chance and is guided toward the furrow by the cortical flow on the dividing cell, and then physically assists the separation of the daughter cells. View Full-Text
Keywords: chemotaxis; cortical flow; cytokinesis; Dictyostelium; midwife chemotaxis; cortical flow; cytokinesis; Dictyostelium; midwife
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Tanaka, Y.; Jahan, M.G.S.; Kondo, T.; Nakano, M.; Yumura, S. Cytokinesis D is Mediated by Cortical Flow of Dividing Cells Instead of Chemotaxis. Cells 2019, 8, 473.

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